Danvers, MA – April 5, 2014 – Cell Signaling Technology, Inc. (CST) of Danvers, MA, announced today that it has introduced a highly specific PD-L1 rabbit monoclonal antibody (mAb). Recent reports have shown PD
Monoclonal antibody is produced by immunizing animals with a recombinant fragment corresponding to residues within the extracellular domain of mouse PD-L1 protein. Background Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regula...
f, A schematic diagram of the PD-L1 Lys263 acetylated peptide and non-acetylated peptide used for immunization to generate the anti-Ac-K263 PD-L1 antibody. g, Dot-blot testing of acetylated and non-acetylated peptides using indicated purified antibodies. h, IB analysis of WCL and anti-HA ...
Cell lysate was fractionated using an isolation kit, as described in the “Materials and Methods” section. The mammospheres of MDA-MB-231 cells were treated with 1 µM JQ1 or 0.5 µM ARV-825 for 24 h. The fractions were analyzed by Western blot with PD-L1 antibody, and subcellular...
(PD-L1) gene is shown in Fig. 3G. Mammospheres were treated with 1 µM JQ1 or 0.5 µM ARV825. ChIP analysis using an antibody against BRD4 and the negative control IgG.HEffect of PD-L1 on mammosphere formation. Cultured MDA-MB-231 cells were treated with siPD-L1 for 2 days. ...
NDR1 (A-8) (sc-365555, Santa Cruz), PD-L1 (#13,684,cst), USP10 (#8501,cst), H3 (#4499,cst) His-tag (AE068, ABclonal), HA-tag (AE008, ABclonal), Myc-tag (AE010, ABclonal).CD25 (ab227834, Abcam). PD-L1 (2B11D11, proteintech). anti-human antibody(Biolegend): PE-CD3...
For EV treatment or PD-L1 blockage, EVs and the anti-mouse PD-L1 monoclonal antibody (mAb) (BE0101-50 mg, BioXcell, Lebanon, NH, USA) were co-injected with cells at the indicated doses. After treatment for 48 h, breast cancer growth and metastasis in zebrafish were observed under...
Chromatin was immunoprecipitated by negative control Normal Rabbit IgG (Cell Signaling Technology#2729) or Flag antibody (1:100, Cell Signaling Technology#14793) at 4 °C with rotation overnight. After eluting chromatin from Antibody/Protein G Magnetic beads and reversing cross-links, the ...
cell immunity, we chose CTLA4 blockade for the combination therapy with RBMS1 depletion in the mouse 4T1 engraftment model. BALB/c mice were inoculated with RBMS1-depleted or control 4T1 cells. When tumors were palpable, mice were treated with control or anti-CTLA4 antibody as indicated (Fig...
Cell lysates were incubated with protein A/G beads and antibody overnight at 4°C. After incubation, the beads were washed with high stringency buffer (15 mM Tris, pH7.5, 120 mM NaCl, 25 mM KCl, 5 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 50 U/ml RNaseOUT, protease ...