When we tested the expansion of DN1 and DN3 cells from the WT, Gnai2fl/flcd4-cre, Gnai2−/−, and Gnai3−/− mice in the OP-9 DL1 culture system, we found a severe defect with the Gnai2−/− DN1 cells (Fig. 1D). They expanded poorly and generated few mature ...
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