Fig. 1: Loss of Pdcd1 enables oncogene-enforced glycolysis in T cells. a, Transgenic ITK-SYK allele with eGFP reporter sequence and Cre-induced excision of the stop cassette. IRES, internal ribosome entry site; TAM, tamoxifen. b, Experimental strategy to explore early molecular events upon ITK...
Phenotypic and transcriptional profiling of regulatory T (Treg) cells at homeostasis reveals that T cell receptor activation promotes Treg cells with an effector phenotype (eTreg) characterized by the production of interleukin-10 and expression of the inhibitory receptor PD-1. At homeostasis, blockade...
a-b,Upper panels, Growth curves of (a) PDL1-sufficient MC-38 tumors or (b) PDL1-sufficient HEPA1-6.X1.1 tumors in control, total PD-L1 knockout, or DC PD-L1 knockout (Clec9a.Cre) mice (a-b, n = 10 per group). Data are representative of two independent experiments.a-b,...
These secondary tumors could represent either skin metastases or tumors that form due to leakiness of the cre-transgene. Consistent with our findings upon tumor rechallenge, mice in which the primary tumors were treated with the tritherapy in addition to BRAF inhibition had significantly reduced ...
(H) T cells from indicated mice were labeled with Cell Trace Violet and incubated for ∼65 hours at a 1:10 ratio with BMDCs pulsed with ovalbumin. Representative of three independent experiments. (I) IFN-γ production by CD4 and CD8 T cell splenocytes from CD4CreSatb1f/f versus Satb...
(25 mg/kg), anti-ILT4 mAb 7B1 (18.75 mg/kg), anti-PD-1 mAb E1A9 (18.75 mg/kg), a combination of 7B1 (18.75 mg/kg) and E1A9 (18.75 mg/kg), or CDX-585 (25 mg/kg) for five weeks. Tumors were measured by caliper three times per week, and mice were euthanized according ...
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