2.Alkindi M, Siminovitch KA, Gupta M, et al.Monoclonal antibodies for the treatment of hypercholesterolemia:targeting PCSK9[J].Can J Cardiol, 2016, 32 (12) :1552-1560 3.Bjorklund M M , Hollensen A K , Hagensen M K , et al. Induction of atherosclerosis in mice and hamsters without g...
2.Alkindi M, Siminovitch KA, Gupta M, et al.Monoclonal antibodies for the treatment of hypercholesterolemia:targeting PCSK9[J].Can J Cardiol, 2016, 32 (12) :1552-1560 3.Bjorklund M M , Hollensen A K , Hagensen M K , et al. Induction of atherosclerosis in mice and hamsters without g...
于是，Bjørklund MM等人利用腺相关病毒（AAV）表达功能获得性PCSK9突变体，开发了一种无种系基因工程的...
转入野生型基因(AAV-hPCSK9FL)可以恢复小鼠体内的PCSK9表达,而转入敲基因的PCSK9Q152H基因,会有大量的pro PCSK9表达,他们是未成熟的PCSK9前体并不能分泌出内置往外。另外又检测了LDLR的染色和蛋白表达,说明利用腺病毒(AAV)转入基因的方法是成功的。 F...
✔AAV-PCSK9造模方法利用小鼠构建动脉粥样硬化的模型，通常用如下方法：包装PCSK9腺相关病毒(rAAV-D377...
3、Kumar S, Kang DW, Rezvan A, Jo H. Accelerated atherosclerosis development in C57Bl6 mice by overexpressing AAV-mediated PCSK9 and partial carotid ligation. Laboratory investigation; a journal of technical methods and pathology. 2017;97(8):935-945. ...
AAV8.2-PPARα virus vector can reduce the plasma lipid level and AS formation in hPCSK9-Tg mice. These finding demonstrate that PCSK9expression notably facilitated AS progression in PPARαmice by increasing plasma lipid concentrations and inflammation. However, overexpression of PPARα can reduce AS...
suggesting that PCSK9 is associated with the loss of structural integrity of the aorta and subsequent imbalanced vasoconstriction7. In addition, infection of C57BL/6 mice with AAV that obtained stable expression of functionally mutated mouse PCSK9 provided a model for rapid enhancement of AngII-induc...
Gut:Pancreas-directed AAV8-hSPINK1 gene therapy safely and effectively protects against pancreatitis in mice. 特异性安全靶向胰腺的AAV8-hSPINK1基因治疗安全有效地改善小鼠胰腺炎。 研究通过构建AAV8-hSPINK1载体特异性安全靶向胰腺,对其他关键器官表现出低趋向性,明确地证明了AAV8-hSPINK1载体在胰腺中的高特...
Gut:Pancreas-directed AAV8-hSPINK1 gene therapy safely and effectively protects against pancreatitis in mice. 特异性安全靶向胰腺的AAV8-hSPINK1基因治疗安全有效地改善小鼠胰腺炎。 研究通过构建AAV8-hSPINK1载体特异性安全靶向胰腺,对其他关键器官表现出低趋向性,明确地证明了AAV8-hSPINK1载体在胰腺中的高特...