If even one of these reagents is missing, the amplification of genetic material will not occur. Each of these reagents and their functions will be discussed in detail in the next several sections of this lesson. Template DNA Template DNA is best defined as the starting material for genetic amp...
and PCR buffer. These reagents are commonly used with difficult samples such as GC-rich templates. Note that use of chemical additives or co-solvents can affect primer annealing, template denaturation, Mg2+binding, and enzyme activity...
Purchase of the instrument includes the right to use the instrument for internal research and to perform services (including the right to report the results of services for a fee) by the purchaser only, but does not convey rights to use any other products, reagents, assays or methods such ...
Modified double-stranded oligonucleotides that have terminal regions on each of their strands, that have a hybrid length of 6-50 nucleotides long, that have a melting temperature Tm of at least 32掳C, and that include 2-4 modifying groups, each covalently attached to a different terminal ...
(robotize if possible) and minimizing the number of pipetting steps. Mix cDNA with qPCR reagents,...
“closed systems”, requiring the use of specific reagents, kits or reaction vessels, produced by the same manufacturer as the PCR machine itself. Apart from often being pricey, this option greatly reduces flexibility in terms of general supply and experiment optimization. This is the case with ...
The resulting assays consist of transcript-specific primer sequences, a reagents protocol as well as instrument settings which are provided in a web-based tool called PCRdrive. PCRdrive was designed to support PCR users in their PCR-related tasks and is equipped with handy functions, components ...
Separate rooms should be set up for reagent preparation, PCR, and product analysis to reduce the risk of contamination. Single-use aliquots of reagents should be used. Disposable plunger pipettors with extra-long pipette tips should be used on a regular basis. Conclusion The use of genotyping ...
Prepare the plate with your own reagents Alternatively, you can prepare your own reagent mixture to be used in the HCP run. The 1x mixture needs to contain a purified amplicon at a concentration of 0.1- 0.3 mM. Ideally, the amplicon has a melting temperature (Tm) close to that of the ...
PCR products and reagents are normally transported on dry ice, but this entails the risk of the dry ice evaporating before packages reach their destination. Long-term ambient storage of nucleic acid samples, PCR reagents, and amplified products is preferentially achieved through lyophilization and air...