PCR (Polymerase Chain Reaction)is an amplification technique that is widely used to generate large amounts of a specific DNA segment using a small amount of starting material, which could be a DNA template or target sequence). There are 5 key basic reagent required to complete a PCR...
You can, under carefully optimized conditions, perform multiplex qPCR to measure the expression of three or four genes simultaneously in a reaction. This can provide huge savings in cost, reagents and time, but the resulting experiments are more complex, and validation bec...
Perform cell lysis and RT-qPCR assays directly from cell cultures with SingleShot™ Cell Lysis RT-qPCR Kits — no RNA purification required. Learn more » Reverse Transcription Quantitative PCR (RT-qPCR) Two methods are available for quantification of gene expression by RT-qPCR: two-step RT...
nationwide distribution network rapidly developed by Randox Laboratories to play a key role in meeting the sudden national demand for Covid-19 PCR tests. At the height of the pandemic emergency, Randox were the only laboratory in the UK and Northern Ireland to process over 100,000 PCR tests pe...
PCR:PCR is a technique used to selectively amplify a region of DNA from a mixed template. Reagents are added to special thin-walled tubes, mixed and centrifuged, loaded in the thermal cycler for the duration of the thermal cycling program, cooled, and evaluated by agarose gel electr...
What are the role of DNA polymerase and RNA polymerase? What is DNA polymerase? What function does it have? What is the role of DNA polymerase I? Why did the use of DNA polymerase from the bacterium Thermus aquaticus allow researchers to add the necessary reagents to tubes in a preprogramme...
As a pioneer in healthcare, we have been committed to improving lives since the company was founded in 1896 in Basel, Switzerland. Today, Roche creates innovative medicines and diagnostic tests that help millions of patients globally.
Voges Proskauer (VP) test;is used to detect the ability of bacteria to produce neutral products like acetoin or 2,3-butanediol. Bacteria are inoculated in MR-VP broth and incubated overnight. Following incubation, VP reagents I and II are added and the color change is observed. Development ...
VL diagnosis As the clinical presentation of VL lacks specificity, con- firmatory tests are required to decide which patients should be treated. Such tests should be highly sensi- tive (>95%) as VL is a fatal condition, but also highly specific because the current drugs used to treat VL ...
With regard to electrophoreses, what is the purpose of the agarose gel, buffer solution, electrical current, cathode (negative probe), and anode (positive probe)? Functions of Gel Electrophoresis Components Gel electrophoresis...