(2007) OligoCalc: An online oligonucleotide properties calculator. Nucleic Acids Res. 35 (Web Server Issue), W43–W46. [13] A. Untergasser, et al. (2007) Primer3Plus: A new improved web interface to the popular Primer3 primer design program. Nucleic Acids Res. 35, (Web Server Issue)...
PCR primer efficiency calculator If you prefer, I have created aPCR primer efficiency online calculator. To use this, simply enter the slope of the line, as determined above, and the calculator will return the primer efficiency value and the amplification factor (E). ...
Phusion DNA 聚合酶的退火规则与许多常见 DNA 聚合酶(如 Taq DNA 聚合酶)不同。为了获得最佳结果,请使用www.thermofisher.cn/tmcalculator上的 Tm 计算器。 仅供科研使用。不可用于诊断程序。 规格 高GC PCR 扩增效果中 聚合酶Phusion高保真DNA聚合酶
1Important!Always use the Tmcalculator on our website atthermofisher.com/tmcalculatorto calculate the Tmof your primers and the recommended annealing temperature. Add the following components to each PCR tube. (For multiple reactions, prepare a master mix ...
The Phusion High-Fidelity PCR Kit contains a sufficient supply of Phusion High-Fidelity DNA Polymerase, Phusion HF and GC Buffers, deoxynucleotides, magnesium chloride, DMSO, and DNA size standard to perform 50 reactions (small) or 200 reactions (large). Control template and primers are provided...
Tm Calculator Automatically calculates the melting and annealing temperaturethrough inputtwo primer sequences Hold At 4ºC Yes, an infinite incubation time at low temperature forovernight working Programwizard Yes, create PCR protocols with only a...
Tm Calculator Yes Single document can be set up a number of cycle Yes Multi-file connection Yes Adjustable thermal cover Yes Power failure protection function Yes Product Dimensions 29cmX24cmX25cm Product Weight 7kg The use of ambient temperature 5 - 40 ℃; relative humidity ≤ 80% ...
Platinum SuperFi DNA 聚合酶的退火规则不同于许多常见的 DNA 聚合酶(如 Taq DNA 聚合酶)。为获得更好的结果,请使用我们网站上的 Tm 计算器:www.thermofisher.cn/tmcalculator。 Platinum SuperFi PCR 预混液配有一瓶单独的 SuperFi™ GC 增强剂,设计用于难以扩增和高 GC 含量的模板 (˃65% GC)。
Tm Calculator FAQs & 问题解决指南 FAQs What are the differences between the various available Phusion®products? My results are not as expected. Where can I find troubleshooting help? What ends will my PCR products have? What does exonuclease activity mean for a DNA polymerase?
The allele frequencies for A (wild type) and G (variant) alleles, were calculated and tested for Hardy Weinberg equilibrium using an online calculator (available at: https://wpcalc.com/en/equilibrium-hardy-weinberg/). Results and discussion Fig. 2 Gel band patterns of OPRM1 gene rs1799971 ...