1. Calculate your average Ct values from each of your replicates/triplicates The first step is to average the technical replicate Ct values. The function in Excel is found below, where the Ct1 and Ct2 values represent the cells for each technical replicate. ...
In the chi-square test, how to calculate (the correct number of parameters and consequently) the correct number of degrees of freedom, without using thechi2goffunction? I have indeed noticed that the number of degrees of freedom was slightly different ...
How to calculate put call ratio? How to interpret the put call ratio? Importance of the put call ratio What is a Put Call Ratio? A put call ratio compares the total number of traded put options to the total number of traded call options. It is used by options traders to understand the...
aI need interplate calibration for my RT-PCR experiment 我需要interplate定标为我的RT-PCR实验[translate] aThe color is abnormal, the panel was scratched 颜色是反常的,盘区被抓了[translate] aThe facility does not pay important attention to the IPA, and disregard to make all the operators waring ...
This allowed us to calculate gene-specific PCR efficiencies for every gene to assure an accurate quantification. The validity of the protocol was demonstrated by means of the study of six genes of the flavonoid biosynthesis pathway. No correlations were found between flower colour and the ...
How does the structure of DNA fit its function, especially as it relates to DNA replication? What is the purpose of adding nucleotides to the PCR tube? How do you calculate how may nucleotides there are in a PCR product obtained after 2 cycles? The product is supposed to include the fowar...
This allowed us to calculate gene-specific PCR efficiencies for every gene to assure an accurate quantification. The validity of the protocol was demonstrated by means of the study of six genes of the flavonoid biosynthesis pathway. No correlations were found between flower colour and the ...
To calculate the reaction efficiency of your assay, you need to set up a 10-fold serial dilution of an undiluted sample with a known amount of template DNA. After running a qPCR, create a standard curve with the log of the starting quantity on the x-axis and the Ct values on the y-...
Free PCR and qPCR design tools! IDT offers several free online tools (SciTools Web Tools) for oligonucleotide design and analysis. These tools contain design engines that use sophisticated formulas that, for example, take into account nearest neighbor analysis to calculate Tm, and allow you to be...
Empty CellDefinitionHow to measure and calculateRemarks and explanation Accuracy The proportion of new tests that give correct results as the reference standard (both positive and negative). (Correct positive + correct negative)/total number [29]. Kappa A statistic method to measure agreement between...