Free PCR primer efficiency Microsoft Excel template Are you still struggling to calculate your PCR primer efficiencies? Well, I have made an Excel worksheet to hopefully help you out. All you have to do is to fill in the Ct values from your replicates and the dilution factor used when making...
It is possible to calculate the expected average fragment size for a given genomic DNA if the percent GC content of the DNA and the recognition sequence of the restriction enzyme are known. For example, in a genome with 50% GC content and no dinucleotide bias, a four-cutter can be expecte...
Multilayer Solutions to Challenges in Blow Molding with PCR For extrusion blow molders, challenges of price and availability of postconsumer recycled resins can be addressed with a variety of multilayer technologies, which also offer solutions to issues with color, processability, mechanical properties ...
Luckily, there’s a direct PCR master mix with a universal annealing feature. That means you can use 60°C as the annealing temperature for your primers. This will set you free from spending a lot of time having to calculate annealing temperatures and run assay...
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Pairwise association tests use statistical methods (e.g., chi-squared test, ANOVA, mutual information) to calculate the correlation between each input feature and the resultant AMR phenotype. The most highly correlated features can be selected as input for model training51. Explainable ML models ...
To calculate the expected effect of a double perturbation on each parameter, fractional changes of the individual perturbations are multiplied, analogous to the way phenotypic growth effects caused by pairwise genetic interactions are assessed36. The error bars are calculated by error propagation of ...
It can calculate metabolic fluxes in a steady state, allowing to predict the growth rate of an organism or the production rate of a metabolite. Genotoxin chemical agent that can damage DNA, causing mutations and possibly cancer. Gnotobiotic model all microorganisms present in and on the animal ...
Background subtracted element signals were used to calculate Cy3:Cy5 ratio. The average of the resulting total Cy3 and Cy5 signal gave a ratio that was used to balance or normalize the signals. Oligonucleotide microarray analysis The HU 6800 microarray was obtained from Affymetrix (Santa Clara, ...
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