PCR Fine-Tuned for Better Plant Disease DetectionThe article reports on the patented procedure called Bio-polymerase chain reaction (Bio-PCR) that was developed by U.S. Agricultural Research Service ...
基本 PCR 包括 3 个步骤：1. 模板 DNA 双链的变性2. 引物与模板 DNA 的每条链的退火3. 引物延伸以合成新的 DNA 链在变性步骤中，模板 DNA 被加热到 94°C 以分离互补链。接下来，降低温度（通常为 50°C 至 65°C）使引物与模板 DNA 上的互补靶标重组或退火。退火温度由2个引物的解链温度(T m )...
Vector-Borne Disease is a kind of infectious disease with arthropods as the transmission vector, which is transmitted to animals and human hosts through bites, mainly including mosquito-borne, tick-borne, mite-borne, lice-borne and flea-borne infectious diseases. Almost everyone has been bitten by...
主要简介 血液外泌体正在成为诊断阿尔茨海默病(AD)等脑部疾病的潜在生物标志物。目前缺乏一种超灵敏的技术来识别血液外泌体中的核心AD生物标志物,以优化生物标志物在临床实践中的效用。在这里,使用DNA偶联抗体开发了一种免疫磁性外泌体聚合酶链...
HFMDis a widespread infectious disease globally, large outbreaks occur often in some countries worldwide. Thousands of people may get infected. Some people, particularly young children, may have severe diseases as viral or "aseptic" meningitis, neural pulmonary edema, or myocarditis that require...
近期,中国兽医药品监察所陈晓春课题组完成的题为“外源性马立克氏病病毒荧光定量PCR检测方法的建立”的研究论文在《中国农业科学》2023年第56卷20期正式发表。 陈晓春课题组从NCBI下载MDV 1、MDV 2和MDV 3各毒株UL19序列,进行核苷酸和氨基...
Swine influenza have diverse clinical presentations and are considered economically crucial disease worldwide. It is very important to establish an effective detection method to help inhibit its spread and decrease the economic loss. The aim of this work reported here was to study a SYBR Green-based...
A reverse transcription nested PCR (RT-PCR) assay for the detection of rubella virus RNA using primers from the E1 open reading frame was established. This assay was found to be sensitive (detecting approximately two synthetic RNA copies and RNA extracted from 0.1 50% tissue culture infective ...
The use of quantitative qRT-PCR assays for detection and quantification of late gametocyte stages has revealed the high transmission capacity of the human malaria parasite, Plasmodium falciparum. To understand how the parasite adjusts its transmission in
8 Buszka, K. et al. Liquid Biopsy Analysis as a Tool for TKI-Based Treatment in Non-Small Cell Lung Cancer. Cells11, doi:10.3390/cells11182871 (2022). 9 Romero, A. et al. ctDNA analysis reveals different molecular patterns upon disease progression in patients treated with osimertinib. Tra...