qualitative nucleic acid detection is sufficient. Other applications, however, demand a quantitative analysis. Real-time PCR can be used for both qualitative and quantitative analysis; choosing the best method for your application requires a broad knowledge of this technology. This section provides an ...
DNA fingerprinting, detection of germs or viruses (especially AIDS), and identification of genetic abnormalities are only a few of the laboratory and clinical applications of PCR. How does PCR work To use PCR to amplify a segment of DNA, the sample is heated until the DNA denatures, or sepa...
What is dPCR used for? Uses of dPCR include: Rare allele detection Absolute quantification of viral load Absolute quantification of nucleic acids Absolute quantification in next generation sequencing Absolute quantification in gene expression How is digital PCR better than traditional PCR? Digital PCR ...
Although each NGS technology is unique, they all utilize a version of the "sequencing by synthesis" method, reading individual bases as they grow along a polymerized strand. This is a cycle with common steps: DNA base synthesis on single stranded DNA, ...
SNP detection Why perform SNP genotyping? Using real-time PCR to perform SNP genotyping About single nucleotide polymorphisms Genotyping is the technology that detects small genetic differences that can lead to major changes in phenotype, including ...
The negative predictive value (NPV) of a test is dependent on the prevalence of disease in the population being tested. Prevalence should be low in individuals attending for elective surgery and, as such, a negative PCR test in this context is likely to have a high negative predictive value....
The implementation of POC testing is useful forvalue-based healthcarewhere there is a need for more convenient diagnosis, accurate monitoring, early detection and the prevention and management of chronic conditions. For example, rapid strep tests can allow a patient to receive earlier treatment and ...
In RT-PCR (reverse transcription PCR), RNA is used as the starting genetic material. The process is almost similar to PCR but has an additional step that allows the detection and amplification of RNA. During the RT-PCR process, the RNA is first reverse transcribed into a...
Detection of HIV-1 DNA in pediatric AIDS brain tissue by two-step ISPCR. Adv Neuroimmunol 1994; 4 : 283-5. Cellular & molecular basis of HIV-associated neuropathogenesis We found that sufficient digestion of cells by proteinase K was crucial for the successful application of indirect ISPCR. ...
Type:Urodynamic Detection System; Group:All; Material:High Tech Materials; Function:Clinical Analysis Instrument; Product Category:Medical Diagnosis Equipment; Product:Clinical Urodynamic System; Pressure Measuring Range:Pressure: -50CMH20(-4.9kpa)~200CMH...