PCR stands forpolymerase chain reaction. It is a revolutionary chemical process that makes it possible to rapidly and exponentially amplify targeted nucleic acid. According to its inventor, American biochemist Kary Mullis, PCR “lets you pick the piece of DNA you’re interested in and have as mu...
DNA fingerprinting, detection of germs or viruses (especially AIDS), and identification of genetic abnormalities are only a few of the laboratory and clinical applications of PCR. How does PCR work To use PCR to amplify a segment of DNA, the sample is heated until the DNA denatures, or sepa...
PCRs have revolutionised the detection of bacteria in clinical samples since their widespread introduction in the 1990s.1 Quantitative PCR (qPCR), also known as specific PCR, involves the targeting of particular bacterial species. The technique uses specific primers (short strands of nucleic acid nee...
An aliquot of the resulting cDNA is then used as a template for multiple qPCR reactions. In the one-step method, RT and qPCR are performed in the same tube. qPCR/Real-Time PCR Instrumentation A real-time PCR detection system consists of a thermal cycler equipped with an optical detection ...
During this process, each unique DNA molecule in the library is bound to the surface of a bead or a flow-cell and PCR amplified to create a set of identical clones. In the case of Ion Torrent technology, a process called “templa...
What is dPCR used for? Uses of dPCR include: Rare allele detection Absolute quantification of viral load Absolute quantification of nucleic acids Absolute quantification in next generation sequencing Absolute quantification in gene expression How is digital PCR better than traditional PCR? Digital PCR ...
Product Name:Fluorescence Quantitative PCR Detection System; Model:Leia-X4; Sample Capacity:96X0.2ml PCR Plate, 12X8-Strip Tubes, 96*0.2ml Sin; Reaction System:5~100UL; Dynamics Range:1-1010; Medical Instrument Registration Certific:G.X.Z. 20...
PCR detection technology is a high-tech detection. In addition, the specificity of new coronal nucleic acid detection brings great difficulties to the detection of samples, followed by a variety of false positive and false negative results. So how to solve these problems has become a difficult pr...
PCRs have revolutionised the detection of bacteria in clinical samples since their widespread introduction in the 1990s.1 Quantitative PCR (qPCR), also known as specific PCR, involves the targeting of particular bacterial species. The technique uses specific primers (short strands of nucleic acid ...
Given the paucity of treatment options, there is a critical need to stop the spread of CPE. However, screening for the presence of CPE in laboratory settings has been challenging. In order to assess the effectiveness of current CPE detection guidelines, we analyzed the meropenem MIC distribution...