It is a simple technique with myriad applications, from criminal forensics to the detection of pathogens.How Does PCR Work? A polymerase chain reaction requires two types of reagents, DNA polymerase and primers, and the process of thermal cycling. These ingredients make possible the chain reaction...
qualitative nucleic acid detection is sufficient. Other applications, however, demand a quantitative analysis. Real-time PCR can be used for both qualitative and quantitative analysis; choosing the best method for your application requires a broad knowledge of this technology. This section provides an ...
each unique DNA molecule in the library is bound to the surface of a bead or a flow-cell and PCR amplified to create a set of identical clones. In the case of Ion Torrent technology, a process called “templating” is used to add library molecules...
What is dPCR used for? Uses of dPCR include: Rare allele detection Absolute quantification of viral load Absolute quantification of nucleic acids Absolute quantification in next generation sequencing Absolute quantification in gene expression How is digital PCR better than traditional PCR? Digital PCR ...
SNP detection Why perform SNP genotyping? Using real-time PCR to perform SNP genotyping About single nucleotide polymorphisms Genotyping is the technology that detects small genetic differences that can lead to major changes in phenotype, including...
DNA fingerprinting, detection of germs or viruses (especially AIDS), and identification of genetic abnormalities are only a few of the laboratory and clinical applications of PCR. How does PCR work To use PCR to amplify a segment of DNA, the sample is heated until the DNA denatures, or sepa...
Based on new trial data regarding immune checkpoint inhibitors (ICIs), the detection of high-grade microsatellite instability (MSI-H) or underlying deficient mismatch repair protein (dMMR) is now becoming increasingly important for predicting treatment response. For the first time, a PD1 ICI (...
there is considerable variability in reported sensitivity and specificity across studies, leading to uncertainty in their clinical utility.#What are the sensitivity, specificity, and diagnostic odds ratios for 16S broad-range PCR followed by Sanger sequencing (16S) and metagenomic next-generation sequencin...
Detection of HIV-1 DNA in pediatric AIDS brain tissue by two-step ISPCR. Adv Neuroimmunol 1994; 4 : 283-5. Cellular & molecular basis of HIV-associated neuropathogenesis We found that sufficient digestion of cells by proteinase K was crucial for the successful application of indirect ISPCR. ...
The implementation of POC testing is useful forvalue-based healthcarewhere there is a need for more convenient diagnosis, accurate monitoring, early detection and the prevention and management of chronic conditions. For example, rapid strep tests can allow a patient to receive earlier treatment and ...