Chen NH, Chen XZ, Hu DM, Yu XL, Wang LL, Han W, Wu JJ, Cao Z, Wang CB, Zhang Q, et al: Rapid differential detection of classical and highly pathogenic North American Porcine Reproductive and Respiratory Syndrome virus in China by a duplex real-time RT-PCR. J Virol Methods. 2009,...
在内参与对照组选择没错的情况下,点击 gene expression 选项,选中你想查看的样品孔的基因表达情况。 好了,这次的 RT-PCR 看图分析就到这里。最后需要注意的是图中的结果还需要输出进行后续的自行运算得出结果。图中的结果只能给出参考。但大致趋势是相同的,如果还有童鞋想听后续如何计算 RT-PCR 的结果及原理的话,...
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甘肃农业大学硕士学位论文抗原捕获反转录聚合酶链式反应(AC-RT/PCR)检测口蹄疫病毒方法的建立姓名:***请学位级别:硕士专业:预防兽医学指导教师:**平;马军武20040601摘要试验采用BHK2l细胞增殖口蹄疫病毒(Foot—and.mouthdiseasevirus,FMDv),经PEG.6000沉淀、差速离心和蔗糖密度梯度离心等方法提纯FMDV146S,免疫动物,制备...
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XZ contributed to the RNA extractions, optimization of the RT-qPCR assay, screening of samples, statistical analysis, and drafting the manuscript. PZ contributed to the sample collection, RNA extraction and drafting of the manuscript. TTM contributed to the sample collection, RNA extraction and ...
The application of microfluidic droplet PCR for single-molecule amplification and analysis has recently been extensively studied. Microfluidic droplet technology has the advantages of compartmentalizing reactions into discrete volumes, performing highly parallel reactions in monodisperse droplets, reducing cross-...