Fig.1. Differential expression of miR-181a-5p and HMGB1 in SCI tissues. (A) SCI animal models were made successfully (red arrow). (B) The morphology of the injured spinal cord (red arrow). (C) Hematoxylin-eosi...
TUNEL staining was used to assess cell apoptosis, and translocation of AIF and EnodoG was observed by immunofluorescence staining and TUNEL staining. Compared with the OGD/R group, the mitochondrial ultrastructure damage were alleviated, the expression levels of OGDH, PDHA1 and COX4 were enhanced ...
Noma T, Yoon YS, and Nakazawa A (1999) Overexpression of NeuroD in PC12 cells alters morphology and enhances expression of the adenylate kinase isozyme 1 gene. Brain Res Mol Brain Res 67:53- 63.Noma T,Yoon Y S,Nakazawa A.Overexpression of NeuroD in PC12 cells alters morphology and ...
After intervened with PI 3K and ERK inhibitors ,the survival rate of PC 12 cells,above mRNA and protein expressions were reversed significantly (P<0.05 or P<0.01);cell morphology was irregular ,the fragments increased ,and the synaptic connections decreased. CONCLUSIONS Sinapic acid can improve ...
5.44.2.1Neuronal Cell Lines A cell line is a general term defining specific population of cells that can maintainin vitroculture for an extended period of time, and is usually clonal meaning that the entire population originated from a single, common, ancestor cell. At present, many neuronal ce...
Connolly J. L., Green, S. A., and Greene, L. A., 1981, Pit formation and rapid changes in surface morphology of sympathetic neurons in response to nerve growth factor,J. Cell Biol.90:176 – 180. ArticlePubMedCASGoogle Scholar
We find that the parent C6 cell line does not require insulin for growth; this may be a result of different requirements of the parent and variant cells, or other components of G2 medium may substitute for the insulin requirement (Michler-Stuke and Bottenstein, 1982a). The morphology of ...
Methods Hydrogen peroxide (H₂O₂) was used to induce PC12 cells to construct an spinal cord injury cell model, with interventions by astragaloside Ⅳ combined with BMSCs-Exos. Laser scanning confocal microscopy was employed to observe the uptake of exosomes by the cells. Flow cytometry was...
The changes of cell morphology was observed by H·E staining, themetabolism vitality was determined by MTT method, the extracellular leakage of LDH was detected byspectrophotometry, the cell survival rate was measured by trypan blue staining counting, the cell apoptosis rate wasdetected by Hochest/...
To determine the relationship between nuclear transcription factor κB (NF-κB) expression in mitochondria and neuronal cell apoptosis after lipopolysaccharide (LPS)-induced injury. Methods The effect of drug administration on PC12 cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl ...