Lipofectamine® LTX 试剂是一种专有的无动物源性制剂,其细胞毒性较低,适用于将 DNA 转染到真核细胞中。本参考资料提供了使用 Lipofectamine® LTX 试剂(货号 15338-100)将质粒 DNA 转染到 PC12 大鼠神经元细胞(ATCC 货号 CRL-1721)中的推荐步骤。转染...
1. Falcon Primaria culture dishes. 2. Culture medium: DME (or F12K) with glutamine, supplemented with 7% heat-deactivated horse serum (56oC for 30 min), 7% fetal calf serum, 50 units penicillin, 50 ug/ml streptomycin, 37oC. 3. ECL matrix (E-C-L cell attachment matrix, Upstate Biotech...
Cell Culture Materials 1. Falcon Primaria culture dishes. 2. Culture medium: DME (or F12K) with glutamine, supplemented with 7% heat-deactivated horse serum (56oC for 30 min), 7% fetal calf serum, 50 units penicillin, 50 ug/ml streptomycin, 37oC. 3. ECL matrix (E-C-L cell attachment ...
为包括脊髓损伤在内的其他神经损伤和疾病的治疗 铁死亡是一种调节性细胞死亡(regulated cell 开辟新的途径. death,RCD)模式,在形态,生物化学和遗传学上有 1 材料 别于其他形式的细胞死亡,具有铁过载,线粒体功能 1.1 细胞株与动物 障碍及脂质过氧化的显著特征[5].研究表明,铁死亡 大鼠 PC12 细胞(批号 20230203)...
Lipofectamine® LTX 试剂是一种专有的无动物源性制剂,其细胞毒性较低,适用于将 DNA 转染到真核细胞中。本参考资料提供了使用 Lipofectamine® LTX 试剂(货号 15338-100)将质粒 DNA 转染到 PC12 大鼠神经元细胞(ATCC 货号 CRL-1721)中的推荐步骤。转染重...
Cell culture and treatment Rat pheochromocytoma PC12 cells were maintained in our laboratory. The cells were cultured in DMEM containing 5% FBS and 5% ES and placed in a water-saturated atmosphere of 5% CO2 at 37 °C. The culture medium was changed every other day. PC12 cells were ...
These results suggest that PC12 in the hormonally defined serum-free media are qualitatively the same as those cultured in serum-supplemented media, and therefore this new culture protocol should enable more precise studies of PC12 cells culture in the absence of confounding unknown factors....
Treatment of PC12 cells with tunicamycin (0.5–10 μg/mL) dose-dependently increased the accumulation of α-syn monomer (19 kDa) and oligomer (55 kDa), and decreased the cell viability. Accumulation of the two forms of α-syn was observed in both the ER and mitochondria with increasing tr...
2.1. Cell culture The experiments were performed on rat pheochromocytoma (PC12) cells transfected with an empty vector (mock transfected) or stably expressing DM1 expanded CUG repeats (CTG90 cells) prepared as previously described [9], [10]. It is worth noting that cells transfected with the ...
Cell culture and treatment PC12 cells were maintained in 1640 supplemented with 10% fetal bovine serum, 100 U/ml penicillin and 100 µg/ml streptomycin in a humidified atmosphere containing 5% CO2atmosphere at 37°C. Cells were seeded at a density of 8×103cells/well in 96-well plates an...