培养体系:RPMI-1640+10% FBS+1%Glutamax+1% Sodium Pyruvate+2.0 ug/mL Puromycin+1%P/S 配套培养基货号:TCH-G380L 传代比例:1:4-1:6,每2-3天换液一次 传代周期:24-32h 培养条件:气相:95%空气+5%CO2,温度:37℃ 冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存 ...
培养体系:RPMI-1640+10% FBS+1%Glutamax+1% Sodium Pyruvate+2.0 ug/mL Puromycin+1%P/S 配套培养基货号:TCH-G380L 传代比例:1:4-1:6,每2-3天换液一次 传代周期:24-32h 培养条件:气相:95%空气+5%CO2,温度:37℃ 冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存 ...
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Stable LBH-overexpressing PC3M-LBH cells and PC3M-NC control cells were obtained via puromycin screening. Cell proliferation was examined using the 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Cell cycle distribution and apoptosis rate were investigated using flow ...
We describe the in vivo ribopuromycylation (RPM) method, which uses a puromycin-specific Ab to fluorescently label ribosome-bound puromycylated nascent cha... MO Seedhom,HD Hickman,J Wei,... - 《Journal of Immunology》 被引量: 3发表: 2016年 The development and validation of the Contextu...
培养体系:RPMI-1640+10% FBS+1%Glutamax+1% Sodium Pyruvate+2.0 ug/mL Puromycin+1%P/S 产品规格:500mL / 200mL / 125mL 产品形态:液体 生物安全检测:细菌、真菌、支原体检测阴性 性能测试:通过细胞培养测试 内毒素含量:<10 EU/mL 储存条件:2-8℃,避光储存 ...