PHAwhichprimarilystimulatesTcellproliferation.Itcanbemodifiedforusewithany ofavarietyofpolyclonalstimulantsthatinteractwiththeTcellantigenreceptoror accessorystructuresTable7.10.1.Usethesupportprotocoltodetermine3Hthymidine incorporation. Immunologic Studies in Humans 7.10.1Copyright 1994 by John Wiley ...
in conjunction with an accompanying support protocol whichcontains methods for pulsing cultures with [3H]thymidine and determining incorporationof [3H]thymidine into DNA. The protocols described here provide an estimate of DNAsynthesis and cell proliferation in an entire cell population, but do not ...
How to Isolate Cells Directly from Whole Blood - EasySep™ Direct Protocol, EasyEights Magnet 51:35 Webinar Streamlining PBMC Isolation with Automation: A Biobank’s Validation Experience 1:01:28 Webinar Optimize the Flow Cytometry Crossmatch Assay: Save Time Without Compromising Sensitivity...
Patient PBMC samples involved in proliferation assay.Yang, LiliWei, YiYang, JiSun, YueShi, WeiminLi, Ming
2013). Although, in T cell proliferation ([H3]-thymidine) and viability (MTT or XTT) assays, 96-well plates are used and only 100 μL of the cell solution are added, resulting in 1–2.5 × 105 cells per well (Martino et al. 2012). T cell culture assays are usually carried ...
本protocol 是一个基本方法,用 CD3 体外刺激小鼠脾 T 细胞和人类外周血 T细胞的增殖,关键参数包括细胞密度、抗体滴度和活化动力学。 一、 小鼠: 用包被的 145-2C11单抗刺激小鼠T细胞;MTT法测细胞增殖 材料 1X 无菌 PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, eBioscience Cat. No. 16-0031...
responses during BC progression. The differentially methylated genes were enriched in immune functions55,56,57, such as regulation of B cell proliferation, regulation of myeloid leukocyte differentiation, and regulation of lymphocyte apoptotic process, supporting the hypothesis that DNA methylation changes ...
responses during BC progression. The differentially methylated genes were enriched in immune functions55,56,57, such as regulation of B cell proliferation, regulation of myeloid leukocyte differentiation, and regulation of lymphocyte apoptotic process, supporting the hypothesis that DNA methylation changes ...
They were then stimulated under standard conditions, and proliferation was determined by [3H]thymidine incorporation between days 2 and 3. (F) TNF release after TGN1412 stimulation of fresh and precultured PBMCs mixed at different ratios during the stimulation assay. Data are mean ± SD of ...
cancers Article Anti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer Minhua Chen 1,2,3,4, Yutong Li 1,2,3,4, Yu Wu 1,2,3,4, Siqi Xie 1,2,3,4, Jie Ma 1,2,3,4, Jingjing Yue 1,2,3,4, Rong Lv 5, Zhigang Tian 1,2,3,4, Fang...