Cellular amino acid uptake is critical for mTOR complex 1 (mTORC1) activation and cell proliferation. However, the regulation of amino acid uptake is not well-understood. Here we describe a role for asparagine as an amino acid exchange factor: intracellu
which are similar to their natural counterpart ATRA, and the competition binding assay has demonstrated that the RAR antagonists could rescue TRC growth inhibited by WYC-209. Furthermore, all human cancer cell lines that we have tested expressed...
转录组学测序(RNA-seq);翻译组学测序(Ribo-seq);Real-Time quantitative PCR (qRT-PCR);RNA荧光原位杂交(FISH);EdU细胞增殖实验(EdU Proliferation Assay);双荧光素酶报告基因系统实验(Dual-Luciferase Reporter Assay);Western Blot实验;免疫荧光技术(Immunofluorescence, IF);...
Furthermore, ASO therapy targeting LINC00673 substantially suppressed breast cancer cell proliferation in vivo. Mechanistically, LINC00673 was found to act as a ceRNA by sponging miR-515-5p to regulate MARK4 expression, thus inhibiting the Hippo signaling pathway. Finally, ChIP assay showed that the...
Moreover, the TUNEL staining assay results indicated that the apoptosis of cells in siUSP18 tumours was substantially higher than that in siNC tumours (Fig. 5c). Fig. 5 Silencing of USP18 inhibited tumorigenicity of human cervical cancer cells in vivo. a & b the tumor volume and weight ...
Assessment of cell proliferation was determined every 72 h using the XTT Cell Proliferation Assay Kit according to the manufacturer's instructions. MSC phenotyping and differentiation assay Cryopreserved MSCs were thawed, washed and cultured for 3 days before phenotyping by using the human MSC ...
GDF15 promotes cervical cancer cell proliferation in vitro and tumor formation in vivo. The proliferation and viability of HeLa and SiHa cells incubated with PBS, HSA(10 ng/ml) or rhGDF15(10 ng/ml) were detected by growth curves (a and b, respectively) and the MTT assay (c and d, re...
Cell Proliferation Assay 2x103 FBXW7 WT/MUT1(R505C)/MUT2(R465C) stably integrated TMK1 cells were seeded into 96-well plates. Cell proliferation was measured the next five days (Day 0-4) by the WST-8 assay (Cell Counting Kit-8, Dojindo). 10 μl of WST-8 solution was added per...
A quantitative high-throughput proliferation assay of 2,816 clinically approved drugs was performed in the NCI-H295R ACC cell line. We validated the antiproliferative effect of candidate compounds in NCI-H295R cells. Further validation was performed in 3-dimensional multicellular aggregates (MCA) of...
we examined proliferation during oxidative stress via the 5-ethynyl-2′-deoxyuridine (EdU) assay. Serum-deprived PC3 (Fig.3a) and DU145 (Fig.3b) cells exhibited proliferation arrest, as expected, and continued after exposure to H2O2for 24 h. Moreover, cell cycle arrest was also observed when...