Flow Cytometry Protocol for FoxP3 on Human Peripheral Blood Mononuclear Cells (PBMC): easy to follow directions describing the step by step experimental procedure.
- 1996 () Citation Context ...alyzed by flow cytometry using a FACScan (BD Biosciences). The acquired data were analyzed with CellQuest software (BD ... E Bottius,L Benmohamed,K Brahimi,... - 《Journal of Immunology》 被引量: 153发表: 1996年 Detection of intracellular antigens in porc...
Objective We aim to develop a protocol for inflammatory and calcium phenotyping of human PBMC by flow cytometry. Methods After thawing PBMC from healthy patients, cell viability is followed by Trypan blue staining to define the optimal time for use. Regarding cell phenotyping, lymphocytes are ...
Detection of Intracellular Factor VIII Protein in Peripheral Blood Mononuclear Cells by Flow Cytometry BioMed Research International PBMCs Cellometer Unspecfied... Trypan blue viability Filbert, Helene October 2012 Serum-free freezing media support high cell quality and excellent ELISPOT assay performance ac...
Figure 3.Correlation of PBMC concentrations measured using three different methods of analysis: flow cytometric cell counting, Scepter™ cell counting, and flow cytometry analysis of fluorescently labeled cells. a Values were derived from the diameter histogram plot ...
incorporationof 3Hthymidine into DNA. The protocols described here provide an estimate of DNAsynthesis and cell proliferation in an entire cell population but do not provide informationon the proliferation of individual cells. To obtain the latter determination of DNA contentby flow cytometry can be ...
Detection of Intracellular Factor VIII Protein in Peripheral Blood Mononuclear Cells by Flow Cytometry BioMed Research International PBMCs Cellometer Unspecfied... Trypan blue viability Filbert, Helene October 2012 Serum-free freezing media support high cell quality and excellent ELISPOT assay performance ac...
The Veri-Cells™ PBMC Kit can be used as positive controls for surface immunofluorescent staining and multi-color flow cytometry assays using the BioLegend surface staining protocol. It can also be used for the detection of intracellular molecules such as Granzyme B, Perforin, Foxp3 and Helios...
Monitor: Monitor cell proliferation and activation markers using flow cytometry. Antigen-Specific T-Cell Activation & Expansion Here's a general protocol: Initial Activation: Co-culture PBMCs with APCs loaded with the specific antigen. Expansion: After initial activation, expand T-cells in the pres...
2013). Flow cytometry can also be used for phenotypic analysis of T cell subsets, frequency determination (Shreffler et al. 2009; Hofmann et al. 2012) and detection of intracellular cytokines (DeLong et al. 2011). Finally, Real-Time quantitative PCR (RT-qPCR) has been largely used for ...