Cell Type Mononuclear Cells Species Human Cell and Tissue Source Peripheral Blood Donor Status Normal View More Data Figures Figure 1.Typical Flow Cytometric Analysis Profile of PBMCs Representative gating strategy of immune cell populations present in PBMCs. Flow cytometry was performed on the peripher...
Single cell RNA sequencing (scRNA-Seq) has revolutionized the analysis of immune cells in humans and animal models1,2,3,4,5. Traditionally, immune cells are characterized by their expression of surface proteins, transcription factors and intracellular cytokines6. Spectral flow cytometry (FACS) and ...
Flow cytometry was conducted in a small group of patients (n = 22). Degranulation was assessed using a lysosomal membrane-associated protein-1 (LAMP-1 or CD107a) marker, which is transported to the CD8 + T cell membrane upon release of cytotoxic granules [62]. The gating ...
Cultured CEM×174 cells were serum- starved and podoplanin expression on viable and apoptotic cells, as determined by forward and sideward scatter, analyzed by flow cytometry (left panel, the histograms were obtained by gating on dead or live cells, as indicated by the arrows in the scatter ...