Flow cytometry analysis is built upon the principle of gating. Gates and regions are placed around populations of cells with common characteristics, usually forward scatter (FSC), side scatter (SSC), and marker expression, to investigate and to quantify these populations of interest. Here we will...
Optimize gating strategy. Even robust manual singlet gating is error-prone and remains a subjective decision point in almost all flow cytometry assays. Imaging can be used to confirm and adjust gates to include only single cells of interest. Here, an experienced user...
Flow cytometry gating strategy for quantification of platelet-derived microparticles in equine platelet samples.Tracy StokolWee Ming YeoDeborah BurnettNicole DeAngelisTeng HuangNikolaus OsterriederJames Catalfamo
This control is particularly important when the expression levels of the markers of interest are low in a multicolor flow cytometry panel. FMO controls should be set up for every fluorophore- labeled antibody in the staining panel, allowing determination of the cut-off between cells that are negat...
Reverse-engineering flow-cytometry gating strategies for phenotypic labelling and high-performance cell sorting. E Becht,Y Simoni,E Coustan-Smith,... - Bioinformatics 被引量: 0发表: 2019年 Fabrication of collagen-coated biodegradable polymer nanofiber mesh and its potential for endothelial cells ...
These findings confirm the precision of FCM for BAL T cell subset ratio analysis and suggest that the optimal gating strategy may be a simple panel using only CD45, CD4 and CD8. 展开 关键词: BAL bronchoalveolar lavage bronchoalveolar lavage FCM flow cytometry flow cytometry ...
Flow cytometry performed on transduced cell lines showed that increasing MOI rates resulted in increased protein levels, plateauing after an MOI of 30. We speculated that, at higher MOI, FVIII production could be impaired by a limiting factor required for proper folding. To test this hypothesis,...
Flow cytometry method for absolute counting and single-cell phenotyping of mycobacteria David A. Barr, Charles Omollo, Mandy Mason, Anastasia Koch, Robert J. Wilkinson, David G. Lalloo, Graeme Meintjes, Valerie Mizrahi, Digby F. Warner & Gerry Davies Scientific Reports volume 11,...
Flow Cytometry Written by:Catriona Paul last updated: April 11, 2022 Gating can be a scary process not What is gating anyway? What you need to know before gating your cells Know the size of your cells Know whether the cells change size under different conditions...
alone. For example, the figure shows an otherwise conventional apoptosis assay using Annexin V and propidium iodide (PI), with added cell imaging to characterize cells in each population, revealing morphologically distinct features. These insights could not have been gained ...