Red cell lysed whole blood (a) was stained for CD3 and CD14 (b) and gates placed around the 3 populations A, B, and C. It can be assumed that population B are granulocytes but no specific marker has been used so a backgating strategy onto a forward and side scatter plot (c) can ...
Gating strategy for analysis of immune cell populations in tumour lesions by flow cytometry.Andrew FreemanJennifer A. BridgePirashanthini MaruthayanarNana H. OvergaardJiWon JungFiona SimpsonTarl W. ProwH. Peter SoyerIan H. FrazerMichael Freeman...
There are a number of factors to consider and incorporate into your FACS staining and gating strategy, including: Compensating for Spectral Overlap The emission spectra of some existing fluorophores are quite large, so the spectral emission of a particular fluorophore can be detected by additional sen...
The most common first gating strategy is to use forward scatter (FSC) and side scatter (SSC) to find viable, single cell events. To find out more about FSC and SSC, see Ryan’s article onflow cytometer parameters. In your experiments you usually want to include only single, viable cells...
Part 3 - GatingStrategy & GatingResults Classes Part 4 - gates Module Part 5 - Session Class Part 6 - Workspace Class Below are more advanced and practical examples for using FlowKit. If you have an example you would like to submit for consideration in this list (preferably with data), pl...
These findings confirm the precision of FCM for BAL T cell subset ratio analysis and suggest that the optimal gating strategy may be a simple panel using only CD45, CD4 and CD8. 展开 关键词: BAL bronchoalveolar lavage bronchoalveolar lavage FCM flow cytometry flow cytometry ...
Summary Chapter Six ("Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating") introduces phenotyping of leukocytes, a major application of flow cytometry. Cells from blood are described along with methods for separating leukocytes from erythrocytes and for using antibodies to ...
CITEViz is intended to be used by biologists familiar with flow cytometry and who can either (1) perform basic single-cell analysis or (2) collaborate in a team with a computational scientists. The input data format for CITEViz is a pre-processed Seurat object, which requires a basic level...
In the studies presented here the adenovirus vector was replication-deficient but the transgene encoding the tumor antigen was under the control of a potent promoter. The strategy was to have transgene-encoded proteins dominate the protein milieu (i.e. the proteins from the viral vector backbone)...
illuminate the relationship between CPCs-Ex and the Akt/mTOR in CVB3-induced apoptosis, proving that CPCs-Ex could suppress the CVB3-induced apoptosis and replication in H9C2 cells and myocarditis rat model, which indicating that CPCs-Ex could be a novel and significant therapeutic strategy for ...