Polymerase complexes possessing PB2-E627K displayed 16.1-fold higher viral polymerase activity when compared to the wild-type virus, which may account for enhanced virulence of this virus. The other two substitutions (HA-N313D and HA-N496S) enhanced binding to both 伪2,3-linked and 伪2,6-...
(MLD50106.5EID50),病毒在小鼠体内的分布情况及各个脏器中的病毒滴度与A/H6N1保持一致;rA/H6N1-627能感染小 鼠,引起小鼠体重下降,但不能引起所有106EID50组小鼠死亡,病毒能在小鼠的肺脏和脑部进行增殖.结论实验结果表明,在H5N1禽流感中发挥重要作 用的PB2-E627K位点并非A/H6N1流感病毒的毒力决定因子.A/H6N1流感...
Biochemical impact of the host adaptation-associated PB2 E627K mutation on the temperature-dependent RNA synthesis kinetics of influenza A virus polymerase complex. J Biol Chem. 2011; 286: 34504-34513.Aggarwal S, Dewhurst S, Takimoto T, Kim B. 2011. Biochemical impact of the host adaptation-...
PB2-E627K and PA-T97I substitutions enhance polymerase activity and confer a virulent phenotype to an H6N1 avian influenza virus in mice. Virology 468C-470C, 207-213.Cheng K, Yu Z, Chai H, Sun W, Xin Y, Zhang Q, Huang J, Zhang K, Li X, Yang S, Wang T, Zheng X, Wang H,...
PB2-E627K and PA-T97I substitutions enhance polymerase activity and confer a virulent phenotype to an H6N1 avian influenza virus in mice. Virology 468-470, 207-213.Cheng K, Yu Z, Chai H, Sun W, Xin Y, Zhang Q, Huang J, Zhang K, Li X, Yang S, Wang T, Zheng X, Wang H, ...
Qinghai H5N1 Mutation PB2 E627K in All Human Isolates - July 9, 2005Genetic Tracking & Scientfic Analysis of Pandemic Influenza & Other Diseases
PB2 E627KHighly pathogenic avian influenza (HPAI) viruses pose a potential human health threat as they can be transmitted directly from infected poultry to humans. During a large outbreak of HPAI H7N7 virus among poultry in The Netherlands in 2003, bird to human transmission was confirmed in ...
Dual E627K and D701N mutations in the PB2 protein of a (H7N9) influenza virus increased its virulence in mammalian models. Sci. Rep. 2015, 5. [CrossRef] [PubMed]Zhu, W. et al. Dual E627K and D701N mutations in the PB2 protein of A(H7N9) influenza virus increased its virulence ...
Virulence of an H5N8 highly pathogenic avian influenza is enhanced by the amino acid substitutions PB2 E627K and HA A149V. Infect Genet Evol. 2017;54:347-54.Wu H, Peng X, Lu R, Xu L, Liu F, Cheng L, Lu X, Yao H, Wu N. Virulence of an H5N8 highly pathogenic avian influenza ...
PB2-292I coupled with 627K also enhances viral transcription and replication in cells, whereas PB2-292I alone did not show the same effect in the H7N9 virus. However, PB2-740A only had a limited prevalence in 2013, and the change from D to A in PB2-740A may have a negative ...