E627KD701NViral pathogenicityMiceBeaglesRecently, canine influenza virus H3N2 (CIV H3N2) has circulated continuously in the dog populations of Asia and the United States (US). As humans have close contact with pet dogs, the circulation of CIV H3N2 is a cause for concern. Previous studies have...
IAV的PB2蛋白对病毒复制至关重要,也是宿主范围的关键决定因素。PB2 E627K替代是大多数人类适应IAV中的主要适应标记,促进哺乳动物细胞中的复制。 此外,发现590S/591R基序可补充2009 H1N1病毒PB2中627K的功能,以允许在人类中复制。此外,627K标记和在较小程度上的701N替代已被描述为在哺乳动物宿主中复制H5N1和H7N...
The PB2-E627K Mutation Attenuates Viruses Containing the 2009 H1N1 Influenza Pandemic Polymerase The swine-origin H1N1 influenza A virus emerged in early 2009 and caused the first influenza pandemic in 41years. The virus has spread efficiently to both ... BW Jagger,MJ Memoli,ZM Sheng,... -...
生的氨基酸替换(PB2:E627K,D701N;NP:N319K) 可以改善禽流感病毒PB2 和NP 蛋白与哺乳动物细 胞α1输入蛋白结合的能力,增强病毒RNA聚合酶在 哺乳动物细胞中的活性,提高禽流感病毒在哺乳动 物细胞中的复制能力和毒力 [10,19,28] 。另外,PB2 蛋白 ...
in PB2 have been reported in different domains of the PB2 protein3,4,9–27. The E627K mutation most potently increases polymerase activity, replication efficiency and pathogenicity of avian IAVs in mammalian hosts3,4,17. However, avian IAVs cumulatively have acquired multiple mutations other ...
(I283M/K526R) in the PB2 and tested their localization. JAK1 co-localized with PB2 appeared in CZ-infected cells (Fig.6c), but this colocalization was not observed in double substitutions (PB2-M283I/R526K-CZ) in the rCZM-infected cells (Fig.7d). In contrast, this colocalization...
In this study, we show that the adaptive mutation PB2 E627K involves an interaction with importin-α by a novel mechanism that does not mediate nuclear transport of viral factors. We found that human importin-α1 and -α7 are required for efficient human- (PB2 627K) but not avian-like...
犬流感反向遗传定点突变E627K甲型流感病毒PB2蛋白627位点突变(E627K)是影响流感病毒宿主适应性和致病性的重要因素之一.本实验室前期研究分离的H3N2亚型犬流感病毒(CIV) GD02株(A/canine/Guangdong/02/2011) PB2蛋白序列的627位点氨基酸为谷氨酸,为研究该病毒株PB2的E627K突变对小鼠致病性的影响,本实验采用反向遗传...
Qinghai H5N1 Mutation PB2 E627K in All Human Isolates - July 9, 2005Genetic Tracking & Scientfic Analysis of Pandemic Influenza & Other Diseases
Polymerase complexes possessing PB2-E627K displayed 16.1-fold higher viral polymerase activity when compared to the wild-type virus, which may account for enhanced virulence of this virus. The other two substitutions (HA-N313D and HA-N496S) enhanced binding to both 伪2,3-linked and 伪2,6-...