目的利用A/H6N1亚型禽流 感病毒的反向遗传平台,评估PB2 E627K对A/H6N1亚型禽流感病毒的致病性,探究A/H6N1流感病毒的致病性分子基础.方法通过A/H6N1亚型禽流感病毒A/Mallard /San-Jiang/275/2007株反向遗传操作系统和点突变技术拯救病毒rA/H6N1和PB2 E627K位点发生突变的rA/H6N1-627,两株拯救病毒分别以101EID50...
IAV的PB2蛋白对病毒复制至关重要,也是宿主范围的关键决定因素。PB2 E627K替代是大多数人类适应IAV中的主要适应标记,促进哺乳动物细胞中的复制。 此外,发现590S/591R基序可补充2009 H1N1病毒PB2中627K的功能,以允许在人类中复制。此外,627K标记和在较小程度上的701N替代已被描述为在哺乳动物宿主中复制H5N1和H7N...
PB2 E627K替代是大多数人类适应IAV中的主要适应标记,促进哺乳动物细胞中的复制。 此外,发现590S/591R基序可补充2009 H1N1病毒PB2中627K的功能,以允许在人类中复制。此外,627K标记和在较小程度上的701N替代已被描述为在哺乳动物宿主中复制H5N1和H7N7AIV的关键。526R是AIV适应哺乳动物的另一个标志物,可能通过...
Dual E627K and D701N mutations in the PB2 protein of A(H7N9) influenza virus increased its virulence in mammalian models . Sci Rep . 2015; 5 : 14170 doi: 10.1038/srep14170Zhu, W. et al. Dual E627K and D701N mutations in the PB2 protein of A(H7N9) influenza virus increased its ...
The PB2 E627K substitution is a dominant adaptation marker in most human-adapted IAVs, facilitating replication in mammalian cells. Furthermore, the 590S/591R motif was found to complement the function of 627K in the PB2 of the 2009 H1N1 virus to allow replication in humans13. In ...
In 2023 in Russia we have isolated two highly pathogenic avian influenza A(H5N1) clade 2.3.4.4b viruses from birds one of which contained an E627K mutation in the PB2 protein. This virus had increased virulence in mice. Limited airborne transmission of the virus with the PB2-E627K ...
More importantly, the E627K mutation has been frequently observed in avian H5N1 viruses crossing the species barrier and causing human death (37, 39). Recent studies found that substituting Glu627 in PB2 with Lys changes its interaction with importin-α1 and importin-α7 as well as the ...
The 2009/2010 pandemic influenza virus (H1N1pdm) contains an avian-lineage PB2 gene that lacks E627K and D701N substitutions important in the pathogenesis and transmission of avian-origin viruses in humans or other mammals. Previous studies have shown that PB2-627K is not necessary because of...
E627KD701NViral pathogenicityMiceBeaglesRecently, canine influenza virus H3N2 (CIV H3N2) has circulated continuously in the dog populations of Asia and the United States (US). As humans have close contact with pet dogs, the circulation of CIV H3N2 is a cause for concern. Previous studies have...
Rapid emergence of a PB2- E627K substitution confers a virulent phenotype to an H9N2 avian influenza virus during adoption in mice. Arch Virol 160, 1267-1277.Sang, X., Wang, A., Chai, T., He, X., Ding, J., Gao, X., et al. (2015a). Rapid emergence of a PB2-E627K ...