本发明提供了一种利用源于患者的肿瘤样细胞簇(patientderivedtumorlikecellcluster,PTC)构建妇科恶性肿瘤细胞系的原代细胞培养方法.PTC是原发性上皮细胞,间质细胞,成纤维细胞,免疫细胞和肿瘤细胞自组装并且增殖的结果,分裂时间短(36天),异质性高.利用PTC构建妇科恶性肿瘤细胞系,在保留细胞系的特性及增殖能力的同时,可...
PDX (Patient-Derived Xenografts)动物模型是指将病人的新鲜肿瘤组织简单处理后移植到免疫缺陷小鼠上,依靠小鼠提供的环境生长,这种模型保留了原代肿瘤的微环境和基本特性,为研究肿瘤的机制、治疗和药物筛选提供了一个非常理想的体内模型。 小鼠肿瘤模型一直以来都是作为医学研究体内实验的最基本的模型,其建立也是日新月异,...
Patient-derived tumor xenograft (PDTX) mouse models are noted to be superior for novel drug discovery and tumor biology studies due to their ability to maintain tumor heterogeneity and their use as real-time individualized patient models. In this review, the authors highlight the utility of PDTX...
This Viewpoint examines the utility of patient-derived tumor xenografts in mice for assessment of cancer therapeutics and physiologic tumor features. ET Liu,CJ Bult,LD Shultz - 《Jama Oncology》 被引量: 0发表: 2016年 Differential expression of 2',3'-cyclic-nucleotide 3'-phosphodiesterase and neu...
Notably, patients derived similar benefit from the addition of atezolizumab to EP in IMpower133 independent of PD-L1 immunohistochemistry or blood-based tumor mutational burden (bTMB) status [27]; however, the bTMB biomarker needs further refinement and validation as there are variable gene panel ...
Patient-derived tumor xenografts (PDXs), in which tumor fragments surgically dissected from cancer patients are directly transplanted into immunodeficient mice, have emerged as a useful model for translational research aimed at facilitating precision med
The establishing of the first cancer models created a new perspective on the identification and evaluation of new anti-cancer therapies in preclinical studies. Patient-derived xenograft models are created by tumor tissue engraftment. These models accurat
High-throughput screening using patient-derived tumor xenografts to predict clinical trial drug response. Nat. Med. 21, 1318–1325 (2015). Article CAS PubMed Google Scholar Galli, R. et al. Isolation and characterization of tumorigenic, stem-like neural precursors from human glioblastoma. Cancer...
Patient-derived xenografts (PDXs) have become a prominent cancer model system, as they are presumed to faithfully represent the genomic features of primary tumors. Here we monitored the dynamics of copy number alterations (CNAs) in 1,110... U Ben-David,G Ha,Y Tseng,... - 《Tumor Biology...
Tumor growth rates derived from data for patients in a clinical trial correlate strongly with patient survival: a novel strategy for evaluation of clinical ... WD Stein,WD Figg,W Dahut,... - 《Oncologist》 被引量: 104发表: 2008年 Tumor Growth Rates Derived from Data for Patients in a Cl...