To analyze and visualize the intracellular trafficking of any secretory protein, we developed the retention using selective hooks (RUSH) system. This assay allows the simultaneous release of a pool of particular secretory proteins from the endoplasmic reticulum and the monitoring of their anterograde ...
The exit of newly-synthesized proteins from the lumen of the endoplasmic reticulum (ER) is the rate-determining step in protein secretion. Only correctly-folded and fully-assembled proteins exit the ER and progress along the secretory pathway. Folding and assembly in the ER are mediated by a va...
Using this procedure we have characterized the transport of glucosylceramide (GlcCer) and the ganglioside GM3 to the plasma membrane. Newly synthesized GlcCer reaches the plasma membrane in 7.2 min, whereas GM3 requires 21.5 min to reach the plasma membrane. Brefeldin A prevents transport of ...
AHA, an artificial amino-acid analogue of methionine permits the capture of newly synthesized proteins through click chemistry followed by mass spectrometry for protein identification22. After 1 day of BL-CFC culture, cells were treated for 6 hours with TGFβ211 or with the vehicle as a ...
42, No. 5, 386-394, May 2010 Induction of the unfolded protein response and cell death pathway in Alzheimer's disease, but not in aged Tg2576 mice Jin Hwan Lee1,2,3,4*, Sun Mi Won1,2,3*, Jaehong Suh1,2,3, Sun Joo Son4, Gyeong Joon Moon1,2,3,4, Ui-Jin Park1,2,3,4...
We then identify a dual-TIR domain gene from this cluster, GhRVD1, which triggers an effector-independent cell death and is induced by Verticillium dahliae. We confirm that GhRVD1 is one of the causal gene for SHZDI1. Allelic variation in the TIR domain attenuates GhRVD1-mediated ...
As hippocampal neurons respond to diverse types of information1, a subset assembles into microcircuits representing a memory2. Those neurons typically undergo energy-intensive molecular adaptations, occasionally resulting in transient DNA damage3–5. Her
Newly synthesized proteins are particularly vulnerable to misfolding and aggregation due to translational errors during their synthesis, as well as the necessity to fold from linear peptide chains into their final native structures (4). To maintain protein homeostasis, cells contain an arsenal of ...
One characteristic feature of β-cells is highly developed endoplasmic reticulum (ER) due to a heavy engagement in insulin secretion. The ER serves several important functions, including post-translational modification, folding, and assembly of newly synthesized secretory proteins, and its proper ...
Cardiac hypertrophy is a common adaptive response to a variety of stimuli, but prolonged hypertrophy leads to heart failure. Hence, discovery of agents treating cardiac hypertrophy is urgently needed. In the present study, we investigated the effects of QF84139, a newly synthesized pyrazine derivative...