C Quantification of the mean TBR of all lesions per mouse in PARPi-FL-injected mice with mean ± SEM. D Comparison of mean fluorescence intensity (MFI) measured in non-injected mice (n = 2) and the detected lesions in PARPi-FL-injected mice (n = 12). One non-injected...
The DNA repair capacity of human cells declines with age, in a process that is not clearly understood. Mutation of the nuclear envelope protein barrier-to-autointegration factor 1 (Banf1) has previously been shown to cause a human progeroid disorder, Né
Poly-ADP-ribose polymerase 1 (PARP1) is one of the main sensors of DNA damage25,26. Upon DNA breaks, PARP1 rapidly recognizes and binds to damaged DNA ends, where it attaches poly-ADP-ribose (PAR) chains onto itself (autoPARylation) and other nuclear proteins near the damaged site. Whil...
DNA damage shuts down genome-wide transcription to prevent transcriptional mutagenesis and to initiate repair signalling, but the mechanism to stall elongating RNA polymerase II (Pol II) is not fully understood. Central to the DNA damage response, poly(ADP-ribose) polymerase 1 (PARP1) initi...
Telomeres are nucleoprotein structures at eukaryotic chromosome termini. Their stability is preserved by a six-protein complex named shelterin. Among these, TRF1 binds telomere duplex and assists DNA replication with mechanisms only partly clarified. Her
which are responsible for cancer cell growth and the removal of DNA damage. ADP-ribosylation is necessary to maintain active transcription, since it ensures an open chromatin structure that allows high acetylation and low histone density. PARP1-mediated modification of BRG1 and EP300 does not affect...
International Journal of Molecular Sciences Article The Selective Acetamidine-Based iNOS Inhibitor CM544 Reduces Glioma Cell Proliferation by Enhancing PARP-1 Cleavage In Vitro Marialucia Gallorini , Cristina Maccallini *, Alessandra Ammazzalorso, Pasquale Amoia, Barbara De Filippis, Marialuigia Fanta...