This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay. 经测试应用 WB, IHC, IF/...
DNA damage shuts down genome-wide transcription to prevent transcriptional mutagenesis and to initiate repair signalling, but the mechanism to stall elongating RNA polymerase II (Pol II) is not fully understood. Central to the DNA damage response,
Assay-dependent - 其他PubMed (Misc) - 查看2篇已发表文章 产品规格 种属反应 Dog,Horse,Human,Mouse,Rhesus monkey,Rat 已发表种属 Dog,Human 宿主/亚型 Mouse / IgG1 分类 Monoclonal 类型 Antibody 克隆号 123 抗原 Recombinant protein derived from the C-terminal region of human PARP protein. ...
Cleaved PARP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, Sample testHost / Isotype Mouse / IgG1 Reactivity human, mouse, rat Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, Sample test Conjugate Unconjugated CloneNo. 4G4C8 Review Product ...
应用范围WB,IHC,ICC/IF,FCM 基因名称PARP1 克隆号1E12 抗体来源Mouse 抗体类型IgG2a 免疫原E.coli-derived human PARP recombinant protein (Position:Q670-R858).Human PARP shares 94% and 95% amino acid (aa) sequence identity with mouse and rat PARP,respectively. ...
(Fig.5i, Supplementary Fig.8b). To assess whether the oxidative DNA damage repair defect in the NGPS cell lines was caused by the presence of the Banf1 A12T protein we depleted Banf1 from NGPS patient cells and control cells and carried out comet assay analysis. This confirmed that ...
To address whether PARP1 targets TOP1ccs for repair, we measured the accumulation of DNA single-strand breaks (SSBs) in wild-type (WT) andPARP1-/-human RPE-1 cells during incubation with camptothecin, using the alkaline comet assay67(Fig.4A–C). Notably, TOP1 degradation via the proteasom...
1 E-H). Comet assay was used to measure the levels of DNA damage. The Comet Assay is a single cell gel electrophoresis assay (SCGE) for simple evaluation of cellular DNA damage. It is a convenient way to screen for general DNA damage, regardless of the source or nature of the damage....
(E) Co-IP/WB assay confirming the interaction between Myef2 and PARP1 and the presence of PARylated Myef2 at the molecular weight of Myef2 (∼55 kD) in D2 primary OLs. (F) qRT-PCR assay for Myef2 in primary OPCs and differentiating OLs. (G) Double IHC of PARP1 and Myef2...
To address whether PARP1 targets TOP1ccs for repair, we measured the accumulation of DNA single-strand breaks (SSBs) in wild-type (WT) andPARP1-/-human RPE-1 cells during incubation with camptothecin, using the alkaline comet assay67(Fig.4A–C). Notably, TOP1 degradation via the proteasom...