Applications Tested: This HLNC4 antibody has been tested by immunoblotting of staurosporine-treated Jurkat cells. This can be used at less than or equal to 0.1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest....
Poly-ADP-ribose polymerase 1 (PARP1) is one of the main sensors of DNA damage25,26. Upon DNA breaks, PARP1 rapidly recognizes and binds to damaged DNA ends, where it attaches poly-ADP-ribose (PAR) chains onto itself (autoPARylation) and other nuclear proteins near the damaged site. Whil...
PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal frag...
Assay-dependent - 其他PubMed (Misc) - 查看2篇已发表文章 产品规格 种属反应 Dog,Horse,Human,Mouse,Rhesus monkey,Rat 已发表种属 Dog,Human 宿主/亚型 Mouse / IgG1 分类 Monoclonal 类型 Antibody 克隆号 123 抗原 Recombinant protein derived from the C-terminal region of human PARP protein. ...
DNA damage shuts down genome-wide transcription to prevent transcriptional mutagenesis and to initiate repair signalling, but the mechanism to stall elongating RNA polymerase II (Pol II) is not fully understood. Central to the DNA damage response,
应用范围 WB,IHC,ICC/IF,FCM 基因名称 PARP1 克隆号 10G9 抗体来源 Mouse 抗体类型 IgG2b 免疫原 E.coli-derived human PARP recombinant protein (Position:Q670-R858).Human PARP shares 94% and 95% amino acid (aa) sequence identity with mouse and rat PARP,respectively. 实际分子量 113KD 成分 50...
3a. Samples collected at the indicated time points were analyzed by WB for PARP1 depletion efficiency (Supplementary Fig. 7A), ChIP against TRF1 to analyze its association to telomeric chromatin and by BromoIP assay to assess the replication fork passage (Fig. 3b, c). Analysis of control ...
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To address whether PARP1 targets TOP1ccs for repair, we measured the accumulation of DNA single-strand breaks (SSBs) in wild-type (WT) andPARP1-/-human RPE-1 cells during incubation with camptothecin, using the alkaline comet assay67(Fig.4A–C). Notably, TOP1 degradation via the proteasom...
(Fig.5i, Supplementary Fig.8b). To assess whether the oxidative DNA damage repair defect in the NGPS cell lines was caused by the presence of the Banf1 A12T protein we depleted Banf1 from NGPS patient cells and control cells and carried out comet assay analysis. This confirmed that ...