目的观察金丝桃苷对人NK细胞体外增殖及杀伤胰腺癌PANC1细胞活性的影响,探讨其可能机制。 方法分离健康人外周血单个核细胞(PBMCs),应用含IL-2的NK细胞培养液加不同浓度(0.3、1.6、8、40、200 μg/ml)的金丝桃苷溶液诱导PBMCs 12 d,以不加金丝桃苷作为对照组。采用台盼蓝染色观察细胞存活数;流式细胞术检测NK...
In recent years, the application of single-cell transcriptomics has unraveled the heterogeneity of CAFs within many cancer types, such as bladder carcinoma (BC) [6], head and neck squamous cell carcinoma (HNSCC) [7], papillary thyroid carcinoma (PTC) [8], and lung cancer (LC) [9]. Furth...
Pan-cancer profiling of tumor-infiltrating natural killer cells through transcriptional reference mappingdoi:10.1038/s41590-024-01884-zThe functional diversity of natural killer (NK) cell repertoires stems from differentiation, homeostatic, receptor鈥搇igand interactions and adaptive-like responses to viral...
Sp1, a transcription factor, regulates essential cellular processes and plays important tumorigenic roles across diverse cancers. However, comprehensive pan-cancer analyses of its expression and potential immunomodulatory roles remain unexplored. Utilizi
1C). Furthermore, our analysis of CCLE data demonstrated distinct PRKCSH mRNA expression levels in 32 tumor cell lines, with the THCA cell line exhibiting the highest levels and the DLBC cell line displaying the lowest levels (Supplementary Fig. 1A–H). Figure 1 Pan-cancer PRKCSH expression ...
增强NK细胞对胰腺癌PANC1细胞的杀伤活性.%Objective To investigate the effect of hyperoside on proliferation and killing activity of NK cells against pancreatic cancer PANC1 cells in vitro,and explore its potential mechanism.Methods Peripheral blood mononuclear cells of healthy donors were isolated,NK...
NK CD56dim cells The amount of CD56 present on an NK cell is indicative of its age and differentiation state; CD56 dim cells are mature NK cells, more commonly found in peripheral blood than secondary lymphoid tissues and have the greatest cytolytic activity.Related...
The clinical correlations of IL32 in pan-cancer were evaluated. The correlation between IL32 and immune cell infiltration was calculated Sangerbox 3.0 (http://www.sangerbox.com) is a web-based bioinformatics analysis platform.We used Sangerbox 3.0, utilizing data from the XCELL database to ...
Cancer-associated fibroblasts (CAFs) are a heterogeneous cell population that plays a crucial role in remodeling the tumor microenvironment (TME). Here, through the integrated analysis of spatial and single-cell transcriptomics data across six common can
Ocular Choroidal Melanoma-1Cells;细胞背景资料:葡萄膜黑色素瘤;女性;细胞传代方法:1:2-1:3传代;每周换液2-3次。;细胞生长特性:贴壁;细胞形态特性:详见细胞说明书;相关细胞有:XuanWei Lung Cancer-05Cells、A375-SMCells、CHP 212细胞 H-1618Cells;细胞背景资料:详见相关文献介绍;细胞传代方法:3-4天换液1次...