Silva教授团队在Chemical Science上发表题为“Phase separation of p53 precedes aggregation and is affected by oncogenic mutations and ligands”的文章,对p53相分离与聚集过程以及相分离的调控进行了研究。 ——相分离与聚集—— 在本研究中,研究人员使用聚乙二醇(PEG)模拟拥挤环境,使用可变温度刺激聚集,研究了多种...
Liquid-liquid phase separation of various transcription factors into biomolecular condensates plays an essential role in gene regulation. Here, using cellular models and in vitro studies, we show the spatiotemporal formation and material properties of p53 condensates that might dictate its function. In ...
Pin1:肽基脯氨酰顺反异构酶1,能特异地识别和结合蛋白质磷酸化的丝/苏-脯氨酸基序,催化磷酸化的丝/苏-脯氨酸肽键发生顺反异构,从而改变磷酸化蛋白质的功能。 相分离:phase separation,是一种最近新提出的蛋白互作模式。在转录调控过程中有重要作用。 p53:主要存在于细胞核,有6个主要的结构域:2×TAD(N-termina...
Intrinsically disordered proteins have the capacity to undergo liquid-liquid phase separation to form membrane-less droplets that act as transcriptional condensates, promote gene expression, and remodel 3D chromatin organization73. However, to what extent p53 has the ability to induce topological changes ...
Intrinsically disordered pro- teins have the capacity to undergo liquid-liquid phase separation to form membrane-less droplets that act as transcriptional condensates, promote gene expression, and remodel 3D chromatin organization73. However, to what extent p53 has the ability to induce topological ...
The SJSA cells were washed with cold PBS once and then lysed in 1 mL of phenol containing TRIzol reagent (Cat. #: 15596018, Thermo Fisher Scientific). For RNA isolation, 200 µL of chloroform was added, and the samples were centrifuged for phase separation. The aqueous phase containing th...
Physiology and pharmacological targeting of phase separation Fangfang Wang Youwei Zhang Journal of Biomedical Science (2024) Oncogenic p53 triggers amyloid aggregation of p63 and p73 liquid droplets Elaine C. Petronilho Guilherme C. de Andrade Jerson L. Silva Communications Chemistry (2024) Am...
These droplets appear to form by liquid-liquid phase separation (LLPS). Unlike amyloid-like prion aggregates, liquid droplets are not stable. They often appear and disappear in the cell in response to protein-specific stress triggers (Boeynaems et al., 2018; Boncella et al., 2020; Buchan...
In brief, following the first phase separation, the aqueous layer was transferred to a new tube. Then, 500 μl of TRIzol and 100 μl of chloroform were added and phase separation was repeated. Isolated RNA was run on a Bioanalyzer 2100 (Agilent) to check for purity. RNA integrity number...
domain (p53(48H, 49H), p53(53S, 54S)), respectively, provided final proof for separation of functions Full size image The formation of DSBs has recently been discovered to represent a frequent byproduct of normal DNA replication, and these DSBs generated at the replication fork are repaired...