NanoTYPE™ is a HLA typing assay using third generation sequencing technology from Oxford Nanopore Technologies (ONT). The kit contains all necessary reagents for the amplification of your sample and comes with our tailored genotyping software, NanoTYPER™. The kit has been designed to be combin...
2019was a year of further innovation and acceleration. In January, the Companyintroduced the 'Field kit'- a lyophilised sample preparation kit that no longer needs a cold chain. Used by scientists sequencing in remote locations, as well as those in the lab, this was another step towards the ...
Designed to ensure optimal device performance and functionality by providing the most up-to-date hardware and software. Find out more Nanopore Service Certified labs Trust your sequencing project to a certified service provider for accredited expertise and high-quality results ...
Nanopore long-read sequencing technology greatly expands the capacity of long-range, single-molecule DNA-modification detection. A growing number of analytical tools have been developed to detect DNA methylation from nanopore sequencing reads. Here, we assess the performance of different methylation-callin...
ONT全长转录组测序是指基于牛津纳米孔公司(Oxford Nanopore Technologies,ONT)三代测序平台进行的全长转录组测序。利用三代测序平台长度长 (long-read)的特性,无需对转录本进行片段化,直接获取某一物种mRNA(或者有polyA尾的lncRNA)5'端到3'端的高质量全长转录组序列信息(图1),可准确识别可变剪接、基因融合、基因...
[8] DUKE J L,MOSBRUGER T L,FERRIOLA D,et al.Resolving MiSeq-generated ambiguities in HLA-DPB1 typing by using the Oxford nanopore technology[J].J Mol Diagn,2019,21(5):852-861. [9] LIND C,FERRIOLA D,MACKIEWICZ K,et al.Next- generation sequencing:the solution for high- resolution...
Oxford, UK:Oxford Nanopore has provided a technology update at its Nanopore Community Meeting, describing a range of product releases and upgrades. These include therelease of “Kit 12", a new kit that includes the latest “Q20+" chemistry and enables “Duplex” sequencing. This is coupled ...
1). The FASTQ file generated by the nanopore whole-genome sequencing consortium was used for the alignment process. The reported and the calculated depth of coverage was found to be ~ 30X. Then, it was down-sampled to 20X and 10X coverage only. The SV call set is used as a ...
Each sample was prepared for sequencing using the ONT SQK-NBD114.96 native barcoding sequencing kit (Oxford Nanopore Technologies, Oxford, UK) for the R10.4.1 flow cells following manufacturer’s instructions with some modifications which can be observed in Supplementary Materials. Data analysis A dia...
Nanopore 8-kb and 20-kb libraries preparation MinION sequencing libraries were prepared according to the nanopore Sequencing Kit protocol SQK- MAP006 or the Low Input Expansion Pack protocol for genomic DNA. Briefly, 100 ng to 1.5 μg of genomic DNA was sheared to approximately 8 Kb with g-...