The technical note demonstrates that the automated preparation of DNA libraries for nanopore sequencing on the Tecan DreamPrep NGS platform using the Ligation Sequencing Kit XL V14 increases consistency and reproducibility compared to manual preparation while freeing staff for higher-value tasks. ...
Return to documentation The final stage of DNA library preparation for the Ligation Sequencing Kit (SQK-LSK109) is to elute the library in Elution Buffer. Typically, after the elution step, the library is mixed with the sequencing buffer and loading beads, then immediately loaded onto the flow...
Oxford Nanopore DNA sequencing Library preparation was carried out using the ligation sequencing kits (Oxford Nanopore Technologies) SQK-LSK109 and SQK-LSK112 for sequencing on R.9.4.1 and the R.10.4 flowcells, respectively. Anaerobic digester and Zymo R.9.4.1 datasets were generated on a MinION...
Ligation Sequencing Kit using 100 ng of sheared and unsheared template DNA. The libraries were run on PromethION flow cells. Panel A: shearing the input gDNA decreases the read lengths that are observed in the subsequent sequencing. Panel B: the flow cell output (Gb) obtained from 100 ng ...
This application note demonstrates that the Ligation Sequencing Kit V14 (SQK-LSK114) can be automated on the DreamPrep NGS platform to increase consistency and reproducibility for high-throughput DNA sequencing. In this application note, you will: ...
This protocol describes how to carry out sequencing of genomic DNA using the Cas9 Sequencing Kit (SQK-CS9109) with enrichment of specific genomic regions using CRISPR/Cas. For users with no previous nanopore sequencing experience, we recommend that a Lambda control experiment is completed first to...
Kit14 將併於 Oxford Nanopore ligation kits 中推出,其中的 native barcoding kits 將於未來擴充至384 barcode(開發中)。此外,快速定序試劑組系列也會進行升級,目標為達到Q20+的準確度,且維持 10 分鐘的簡單建庫流程。 短片段去除試劑組:該試劑可移除長片段建庫應用中的短片段核酸,預計於 6 月可搶先體驗。
What type and how much starting material do I need to use when preparing libraries using the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing? Are the reagents supplied in the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing sufficient to constru...
一旦生成了足够的数据,例如达到覆盖度目标,可以立即停止运行。OxfordNanopore提供高度灵活、可扩展的长读长RNA测序方法。在测序中,RNA直接测序试剂盒(DirectRNASequencingKit)提供了对全长非扩增RNA链进行测序的唯一方法,无需转换为cDNA,可进行序列以及碱基修饰的分析。我...
Oxford Nanopore Technologies provides open access to a range of nanopore sequencing datasets through its initiative hosted on Amazon Web Services (AWS), called‘ont-open-data’. This initiative allows researchers worldwide to explore and utilise extensive sequencing data to enhance their genomic studies...