CD8+T细胞和肿瘤细胞共培养 Day1:接上一篇CD8+T激活,按1 : 1的比例,使用CD3/CD28激活剂(提供第一第二信号)刺激CD8+T细胞,并将其培养在含10% FBS、1%青链霉素双抗生素和10ng/ml IL-2(激活后T细胞才会表达IL-2受体)的DMEM/1640完全培养基中。调整细胞密度为1×106 cells/ml,每2 - 3天换液。 Day7-10...
OT-1小鼠是体内CD8+T特异性识别OVA OT-11小鼠是体内CD4+T特异性识别OVA 表达OVA的肿瘤打给小鼠,再给小鼠OT-1的CD8+T,相当于给小鼠肿瘤特异性T细胞。 OT-I:(CD8+---ovalbumin 257-264 ) These mice containtransgenicinserts for mouse Tcra-V2 and Tcrb-V5 genes. The transgenic T cell receptor was d...
OT-I CD8+ T cells persist within the tumor but also in the lymphoid organs and the periphery.Jérémie, MénagerJeanBaptiste, GorinCatherine, MaurelLucile, DrujontSébastien, GouardCedric, LouvetMichel, ChérelAlain, FaivreChauvetAlfred, Morgenstern...
OT-II和OT-I小鼠都是T细胞受体（TCR）转基因鼠。OT-II小鼠的CD4 T细胞可以识别OVA抗原（OVA-323-339），而OT-I小鼠的CD8 T细胞可以识别OVA抗原（OVA-257-264）。这些小鼠常被用作研究免疫反应和疫苗的模型。
(DC/T比例分别为1/20和1/5)与CD8+ T细胞共培养,刺激CD8+ T细胞增殖分化.于共培养不同时间收集细胞,流式细胞术测定CD8+ T细胞的数量、分裂速度、细胞表面活化分子的表达丰度,碘化丙叮(PI)染色测定细胞活力.[结果] 在任一DC/T比例下,SIINFEKL浓度过低,均不能引起CD8+ T细胞的充分增殖,而高于CD8+ T细胞...
CD8+ T cells (OT-I cells). That is, the CD8 T cells of this mouse primarily recognize OVA257-264 when presented by the MHC I molecule. Immune response dynamics can be studied by in vivo adoptive transfer or in vitro co-culture with cells transgenic for ovalbumin or by direct administrati...
These experiments demonstrate that the ability of T cells to respond to the low-affinity antigen is dependent on the binding of CD8α to the MHC-I. Therefore, we postulate that the increased CD8α and CD8β expression observed on the surface of OT-1 miR-155 contributes to the better ...
Transgenic TCR mice are often used experimentally as a source of T cells of a defined specificity. One of the most widely used transgenic TCR models is the OT-1 transgenic mouse in which the CD8+ T cells express a TCR specific for the SIINFEKL peptide of
CD8 peripheral T cell ratio,and lymph node T cells demonstrate a dose-dependent proliferative response to the specific ovalbumin ligand.These transgenic mice are useful for studying in vivo T cell biology such as TCR-ligand interactions,T cell activation,thymic selection,cross-presentation of antigen...
The Size of the CD8 T Cell Response to CMV is Linked to Advanced Memory Differentiation of T Cells Specific for CMV but Also Other AntigensThe Size of the CD8 T Cell Response to CMV is Linked to Advanced Memory Differentiation of T Cells Specific for CMV but Also Other Antigensalternative...