For analysis at the KIT locus, cells were lysed using QuickExtract DNA Solution (Lucigen) and amplicons were generated using 15 cycles of PCR to introduce Illumina sequencing primer binding sites and 0-8 staggered bases to ensure library diversity. After reaction clean-up using ExoSAP-IT kit (...
In transcription, information from a DNA sequence is converted into a molecule of messenger RNA, or mRNA. Answer and Explanation: The mRNA strand formed from transcription of this DNA strand would be A C U G C A U U G A C G U ...
Mitochondrial genomes are a valuable source of data for analysing phylogenetic relationships. Besides sequence information, mitochondrial gene order may add phylogenetically useful information, too. Sipuncula are unsegmented marine worms, traditionally p
This filtering step was performed to remove the reads with connectors, the reads showing a quality score below 20 (Q < 20), the reads containing a percentage of uncalled bases (“N” characters) equal to or greater than 10%, and repeated sequences. A combination of de novo and reference...