Transfer RNA is uniquely enriched with modified bases. A large body of information has accumulated about the occurrence, nature and distribution of modified bases in tRNA. But similar investigations on the enzymes involved in this post-transcriptional modification have been hampered by the instability ...
Um34 in selenocysteine tRNA is required for the expression of stress-related selenoproteins in mammals Selenium is an essential micronutrient in the diet of mammals and has many health benefits. Selenium-containing proteins are responsible for most, if not all, of these benefits. This element is ...
Identification of direct targets and modified bases of RNA cytosine methyltransferasesCytosinetRNA MethyltransferasesRNASequence Analysis, RNAImmunoprecipitationBase SequenceBinding SitesMolecular Sequence DataThe extent and biological impact of RNA cytosine methylation are poorly understood, in part owing to ...
4, 3065. Role of Hypermodified Bases in Transfer RNA. Solution Properties of The hypermodified dinucleoside monophos-phates, uridylyl(3'-5')-N-[9-(~-~-ribofuranosyl)purin-6-ylcarbamoyl]threonine (Upt6A), adenylyl(3'-5')-N6-(A2-isapenteny1)adenosine (ApihA), adenylyl ( 3'-5')-N6...
When applied in a human cell line to the RNA methyltransferases DNMT2 and NSUN2, Aza-IP enabled >200-fold enrichment of tRNAs that are known targets of the enzymes. In addition, it revealed many tRNA and non-coding RNA targets not previously associated with NSUN2. Notably, we observed a...
2'-O-Methyl RNA Bases 2'-O-Methyl RNA is a naturally occurring modification of RNA found in tRNA and other small RNAs that arises as a post-transcriptional modification. Oligonucleotides can be directly synthesized that contain 2'-O-Methyl RNA. This modification increases Tm of RNA:RNA duplex...
Establishes the modified wobble base inosine in yeast transfer RNA Ile as a positive determinant for aminoacylation. Indication that inosine is a determinant for yeast isoleucyl-tRNA synthetase; Yeast's composition of two isoleucin tRNA; Determinants for isoleucine specificity; Role of the minor tRNA...
Through AM1 calculations we show that, when the first uracil of codon UUC binds to A36 of anticodon GAA of tRNA , dramatic conformational variations occur in the side chain of the hypermodified Y base in position 37. The H-bond found, involving one of the Y side-chain carboxylic oxygens...
microorganisms Review Transfer RNA Modification Enzymes from Thermophiles and Their Modified Nucleosides in tRNA Hiroyuki Hori * , Takuya Kawamura, Takako Awai, Anna Ochi, Ryota Yamagami, Chie Tomikawa and Akira Hirata Department of Materials Science and Biotechnology, Graduate School ...
Wyosine and its derivatives are highly modified, acid labile tricyclic bases found at position 37 of tRNAPhe in archaea and eukarya. The formation of the common 4-demethylwyosine structural feature entails condensation of pyruvate and N-methylguanosine catalyzed by TYW1. This review will fo...