For analysis at the KIT locus, cells were lysed using QuickExtract DNA Solution (Lucigen) and amplicons were generated using 15 cycles of PCR to introduce Illumina sequencing primer binding sites and 0-8 staggered bases to ensure library diversity. After reaction clean-up using ExoSAP-IT kit (...
How does RNA polymerase know which strand of DNA to use as template? Which nucleotide bases are found in RNA? What are the pairings for the bases in RNA and DNA? Problem 1. Convert the DNA into a mRNA. Decode the mRNA into a seq...
There seems to be a correlation between the physical size of co-expression clusters and organism complexity, ranging from a few kilobases in yeast to several megabases in mammals. Many small clusters might be the result of shared regulatory elements. ...
That is, these methods identify which bases are single or double stranded but do not directly reveal the counter-parties in each base pair (Lu and Chang, 2016). Inferring transcriptome structure in living cells is especially challenging, due to the presence of long-range structures, pseudoknots,...
anoplonyxencodes an additional tRNA gene, bringing its total gene count to 38. The base composition of the mitogenomes of these five species exhibited a higher proportion of adenine-thymine (AT) bases. Six start codons and four stop codons were identified across the five species. Analysis of...
Mitochondrial genomes are a valuable source of data for analysing phylogenetic relationships. Besides sequence information, mitochondrial gene order may add phylogenetically useful information, too. Sipuncula are unsegmented marine worms, traditionally p
Long-read sequencing platforms (or third generation) from Pacific Biosciences (PacBio, Menlo Park, CA, USA) and Oxford Nanopore Technologies (ONP, Oxford, United Kingdom) have significantly decreased in cost [53]. With reads in the tens of thousands of bases in length, these technologies are...
This filtering step was performed to remove the reads with connectors, the reads showing a quality score below 20 (Q < 20), the reads containing a percentage of uncalled bases (“N” characters) equal to or greater than 10%, and repeated sequences. A combination of de novo and reference...
Recent progress in second-order NLO ionic organic crystal materials is reviewed in this article. Research has shown that the second-order nonlinear optical properties of organic crystal materials are closely related to their molecular structures. The basic structures of ionic organic conjugated molecules...
SS symmetry Article Research on Collaborative Order Decision-Making Method for Symmetric Participants in Complex Shipbuilding Projects Xuezhang Mao 1,*, Jinghua Li 2,*, Boxin Yang 2,* and Hui Guo 1 1 College of Shipbuilding Engineering, Harbin Engineering University, Harbin 150001, China; hui0625...