This study developed and tested a dual-target (ORF1ab and N gene) one-step nested RT-qPCR (DTO-N-PCR) to detect SARS-CoV-2. Ten-fold serial dilutions of mixed synthetic DNA from SARS-CoV-2 ORF1ab and N gene were used as templates to test the sensitivity of DTO-N-PCR. Its ...
The combination of in vitro fertilization (IVF) with PCR technologies enables diagnosis of single gene defects for preimplantation genetic diagnosis. This has been accomplished by two-step nested PCR, or PEP-PCR followed by nested PCR processes. To improve the detection of single cell gen...
The products of the lysis program were used as tem- plates in a nested PCR analysis. All operations were carried with care to prevent contaminating the samples. Genotyping The genotypes of mutant mice were determined by PCR of genomic DNA extracted from tails. ExTaq was activated at 95 °C ...
免疫捕获RT-PCR 1. In this study,based on the previously reported viral coat protein(CP)gene sequences,specific primers for ORSV and CyMV were designed,and four methods,including ELISA,RT-PCR,nested RT-PCR and IC-RT-PCR were performed for molecular detection of the two viruses. 本研究根...
Nested PCR assay revealed successfully targeted insertion of DOI in all the 8 blastocysts (Figure 4B and C) with targeted insertion efficiency of 21.6% injected zygotes that developed into blastocysts (3.9% of total injected zygotes) (Figure 4D). Figure 4 Production of multiple targeted Tg ...
The application of one-step RT-PCR has a greater advantage over the nested RT-PCR because it eliminates the possibility of cross-contamination between samples and reduces the turnaround time. Comparison of sensitivities of the rRT-PCR and cRT-PCR assays Using serial decimal dilutions (10−1 ...
We used this multiplex PCR to identify haemosporidian infections in 250 adult domestic pigeons (Columba livia) in Turkey. All samples were also screened by microscopy and a widely used nested PCR to compare with the results of multiplex PCR, to detect low levels of parasitemia, and to ...
The VP2 region was amplified in a semi-nested PCR. cDNA synthesis and the first round of PCR were carried out using a OneStep RT-PCR kit (QIAGEN) and primers AM11, AM12, AM31, and AM32 (Nasri et al., 2007), and the second round of amplification was carried out using primers AM...
逆转录环介导等温核酸扩增技术 1. Evaluation of Reverse Transcription Loop-mediated Isothermal Amplification for Detection of Avian Influenza A H5N1 Virus; 逆转录环介导等温核酸扩增技术(RT-LAMP)在H5N1禽流感病毒基因检测中的应用6) reverse transcription-nested PCR(RT-nPCR) 逆转录巢式PCR法补充...
Then, the PCR product was nested using the following primers and 1st PCR product as a template. pPDX1-seqF: 5′-GGCAGTCATGAATGGCGAGGAGCAGTACTACG-3′ pPDX1-seqR2: 5′-CCGGTATGCTTTAGTCCGACTCTGCCTTATCCAC-3′ Amplicon 543 bp. PCR products were cloned into the pCR-Blunt II-TOPO ...