The one step nested PCR method could detect latent infection of P. sparsa in both dormant and active plant growth at 0.4pg genomic DNA. The most reliable detection was achieved from crown or root tissues. For surety of the infection status, replicate plant tissues should be assessed by PCR ...
深入研究「Cost-effective one-step PCR amplification of cystic fibrosis ΔF508 fragment in a single cell for preimplantation genetic diagnosis」主題。共同形成了獨特的指紋。 引用此 APA Standard Harvard Vancouver Author BIBTEX RIS Tsai, Y. H. (1999).Cost-effective one-step PCR amplification...
1) one-step ic-rt-pcr 免疫捕获一步RT-PCR例句>> 2) IC-RT-PCR 免疫捕获RT-PCR 1. In this study,based on the previously reported viral coat protein(CP)gene sequences,specific primers for ORSV and CyMV were designed,and four methods,including ELISA,RT-PCR,nested RT-PCR and IC-RT-...
www.nature.com/cr One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs Erwei Zuo1, *, Yi-Jun Cai1, *, Kui Li1, *, Yu Wei1, 2, 4, *, Bang-An Wang3, *, Yidi Sun6, Zhen Liu1, 8, Jiwei Liu1, Xinde Hu1,...
In this study, new one-step real-time RT-PCR and nested RT-PCR assays were developed to detect HAstVs. HAstVs were identified in 46 (8.75%) of 526 stool samples in Jiangmen City over 1year, including 43 (9.15%) of 470 children and 3 (5.4%) of 56 adults, and HAstV-1 was the ...
Nested PCR assay revealed successfully targeted insertion of DOI in all the 8 blastocysts (Figure 4B and C) with targeted insertion efficiency of 21.6% injected zygotes that developed into blastocysts (3.9% of total injected zygotes) (Figure 4D). Figure 4 Production of multiple targeted Tg ...
The application of one-step RT-PCR has a greater advantage over the nested RT-PCR because it eliminates the possibility of cross-contamination between samples and reduces the turnaround time. Comparison of sensitivities of the rRT-PCR and cRT-PCR assays Using serial decimal dilutions (10−1 ...
A multiplex-polymerase chain reaction (PCR) assay based on one-step amplification and detection of two different mycobacterial genomic fragments was design... DH Shah,R Verma,CS Bakshi,... - 《Fems Microbiology Letters》 被引量: 101发表: 2002年 Evaluation of a polymerase chain reaction-based ...
We have updated an enterovirus assay and combined it with a previously published assay for human parechovirus resulting in a multiplex one-step RT-PCR assay. The multiplex assay was validated by analysing the sensitivity and specificity of the assay compared to the respective monoplex assays, and...
Article: A novel one-step multiplex PCR protocol to detect avian haemosporidian parasites in the subgenus Haemoproteus (Kruse, 1890) used to quanti