Saccharomyces cerevisiae remains an ideal organism for studying the cell biological roles of lipids in vivo, as yeast has phospholipid metabolic pathways similar to mammalian cells, is easy and economical to manipulate, and is genetically tractable. The availability of isogenic strains containing specific...
Structure and function of enzymes in metabolic pathways Role of lipids in cell membrane structure and function Biochemical mechanisms of DNA repair Metabolic pathways in cancer cells: targets for drug development Protein folding and its implications in diseases Biochemistry of hormone action in human phys...
Glycosphingolipids (GSLs) are a family of bioactive lipids that in addition to their role in the regulation of structural properties of membrane bilayers have emerged as crucial players in many biological processes and signal transduction pathways. Rather than being uniformly distributed within membra...
Endogenously, they can be formed through: (1) phosphorylation, catabolism and/or fragmentation of fructose from polyol pathway36,43; and (2) the nonenzymatic reaction of reducing sugars with free amino groups of proteins, lipids and nucleic acids.39 In the first mechanism, fructose can be ...
(f) After treatment with 10 μM 4-en-3-one for indicated time, membrane lipids were extracted from A549 cells and determined using TLC analysis. The free cholesterol content was measured using free cholesterol Assay kits. Cho represented as cholesterol. Data represent means ± S.D. of at ...
Improved one dimensional thin layer chromatographic technique for polar lipids. J. Chromatogr. 332, 391–395 (1985). Article Google Scholar Laloi, M. et al. Insights into the role of specific lipids in the formation and delivery of lipid microdomains to the plasma membrane of plant cells. ...
To determine the orthology of sterol 14-demethylase (P45014DM), the only known P450 enzyme distributed widely in eukaryotes with a conserved metabolic role, the full-length amino acid sequences of rat and human P45014DMs were determined from the cloned cDNA sequences, and compared with those ...
Elevated levels of the aldehydes hexanal and heptanal could be the result of increased membrane lipids oxidation by the large amount of radicals produced in tumour cells (Guadagni et al., 2011). Ethyl acetate most probably results from an esterification reaction involving acetic acid and ethanol. ...
Whole-transcriptome analysis and western blotting of sauchinone-treated HepG2 cells demonstrated that sauchinone regulated genes relevant to cholesterol metabolism and synthesis. In particular, it was found that the expression of proprotein convertase su
Accumulation of neutral lipids in β-Clu-treated cells We have studied the effect of extracellular treatment of α-Clu and β-Clu on the intracellular accumulation of lipids in C2C12 cells. Nile red, a selective probe for accumulation of intracellular lipid droplets, becomes strongly fluorescent upo...