Genome Biology (2020) Evaluation of mutation rates, mosaicism and off target mutations when injecting Cas9 mRNA or protein for genome editing of bovine embryos Sadie L. Hennig Joseph R. Owen James D. Murray Scientific Reports (2020)Download...
zurletrectinib increased brain penetration also translated into improved activity against brain tumors compared to the other next-generation agents, we tested the in vivo activity of zurletrectinib in mouse glioma orthotopic xenografts harboring the Trka G598R or the Trka G598R/G670A mutation. ...
PCR serves as a very convenient detection method, but any mutation that destroys a primer annealing site leads to false-negative results [80]. This problem can be partially solved if the primers are moved away from the DSB site as far as possible [81]. However, PCR am...
In recent decades, scientists around the world induced random mutations, mainly in crop plants, to widen the mutation spectra to be used for extensive screening for varieties useful for agriculture and science (2). As mutagens, ethyl meth-anesulfonate, radiation, Agrobacterium tumefaciens-mediated T...
Semantic Mutation Testing (SMT) is a technique that aims to capture errors caused by possible misunderstandings of the semantics of a description language. It is intended to target a class of errors which is different from those captured... H Dan,RM Hierons - International Conference on Software...
identifying most off-targets with mutation rates superior to 0.1 %, while the number of false positives can be largely reduced with a cutoff on the off-target score. We also evaluate on-target efficiency prediction algorithms against available datasets. The correlation between the predictions and th...
Examples include the inactivation of mutated oncogenes or mutation reversion of tumor suppressor genes. The technology has also been used to enhance the expression of tumor suppressor genes. Another recent progress is the CRISPR-based prime editing technology, which can generate insertions, deletions, ...
关键词: gene detection gene quantification high-throughput screening mutation detection padlock probes DOI: 10.3109/07853899709007487 被引量: 30 年份: 1998 收藏 引用 批量引用 报错 分享 全部来源 免费下载 求助全文 全文购买 Taylor & Francis Semantic Scholar Informa Healthcare NCBI Europe PMC 查看更多 ...
We found that the same mutation could have different transactivation activities when tested with different target binding sites and in different cell lines. The differential transactivation activities of the SOX10 mutants appeared to correlate with the intestinal and/or neurological symptoms presented in ...
(Supplementary Fig.7D). We observed little difference induced by CHX exposure in LKB1 mRNA abundance in the ATI-associated cells when compared to parental cells suggesting that NMD is not acting on the mRNA with an ATI-provoking mutation (Supplementary Fig.7D). On the other hand, in the ...