3 Mutation types and gene expression of off-target RNA SNVs. a, Distribution of mutation types in each repeat of all groups. The number in each cell indicates the percentage of a certain type of mutation among all mutations. b, Expression of genes containing overlapping off-target RNA SNVs...
3 Mutation types and gene expression of off-target RNA SNVs. a, Distribution of mutation types in each repeat of all groups. The number in each cell indicates the percentage of a certain type of mutation among all mutations. b, Expression of genes containing overlapping off-target RNA SNVs...
Off-target RNA mutationBase editors (BEs) is a powerful tool that could edit the genome efficiently without inducing DNA double-strand breaks (DSBs). Recently, off-target DNA and RNA mutations of BEs were found, rasing concerns about its precision and safety. Here we discuss some strategies ...
Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis. Nature 571, 275–278 (2019). Article CAS PubMed Google Scholar Gehrke, J. M. et al. An APOBEC3A–Cas9 base editor with minimized bystander and off-target activities. Nat. Biotechnol. 36, 977–982 (...
Only one off-target indel mutation was identified from the WGS data and validated by Sanger sequencing. In addition, we found 243 newly generated off-target sites caused by genetic variants between the Thompson Seedless cultivar and the grape reference genome (PN40024) but no true off-target ...
RNA-guided endonucleases (RGENs), derived from the prokaryotic adaptive immune system known as CRISPR/Cas, enable targeted genome engineering in cells and organisms. RGENs are ribonucleoproteins that consist of guide RNA and Cas9, a protein component originated from Streptococcus pyogenes. These enzymes...
Although RNA-guided genome editing via the CRISPR-Cas9 system is now widely used in biomedical research, genome-wide target specificities of Cas9 nucleases remain controversial. Here we present Digenome-seq, in vitro Cas9-digested whole-genome sequencing, to profile genome-wide Cas9 off-target effect...
Site-directed RNA editing (SDRE) is a general strategy for making targeted base changes in RNA molecules. Although the approach is relatively new, several groups, including our own, have been working on its development. The basic strategy has been to couple the catalytic domain of an adenosine...
The success of the CRISPR/Cas9 genome editing technique depends on the choice of the guide RNA sequence, which is facilitated by various websites. Despite the importance and popularity of these algorithms, it is unclear to which extent their predictions
Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis. Nature 571, 275–278 (2019). Article Google Scholar Rees, H. A., Wilson, C., Doman, J. L. & Liu, D. R. Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci. Adv...