一个最小的TPP1 OB-fold通过阻止端粒酶接近端粒上的同源结合位点来抑制端粒的维持。我们确定了与端粒酶相关的TPP1 OB fold中的特定环残基以及TERT中的关键残基,包括肺纤维化患者亚组中的突变残基。这些数据定义了端粒维持所需的端粒酶-TPP1相互作用的潜在界面并表明端粒酶相关疾病中端粒酶募集缺陷。 介绍 通过端粒酶将...
( 2003 ) OB-fold: growing bigger with functional consistency . Curr Protein Pept Sci 4 : 195 – 206 .Agrawal V, Kishan KV. OB-fold: growing bigger with functional consistency. Curr Protein Pept Sci 2003;4:195-206.Agrawal, V.; Kishan, K.V. OB-fold: growing bigger with functional ...
The widespread oligonucleotide/oligosaccharide-binding (OB)-fold recognizes diverse substrates from sugars to nucleic acids and proteins, and plays key roles in genome maintenance, transcription, translation, and tRNA metabolism. OB-containing bacterial Trbp and yeast Arc1p proteins are thought to recogniz...
OB-FOLD USED AS SCAFFOLD FOR ENGINEERING NEW SPECI
摘要: OB-fold and RGG domains are dispensable for RHA-stimulated HIV-1 production.OB-fold and RGG domains are not required for activation of HIV-1 transcription by RHA.OB-fold facilitates the generation of HIV-1 6D RNA.关键词:HIV-1 RNA helicase A OB-fold Transcription Splicing ...
Here, we identify a REV7 complex with FAM35A and C20ORF196, which is distinct from the Polζ complex of REV7. FAM35A resembles a telomere-protecting protein, POT1, in that both proteins contain three OB-fold domains and bind single-strand DNA (ssDNA). Cells lacking either FAM35A or ...
BLAP18/RMI2 contains a putative OB-fold domain, and several lines of evidence suggest that it is essential for BTB complex function. First, the majority of BLAP18/RMI2 exists in complex with Topo IIIalpha and BLAP75/RMI1. Second, depletion of BLAP18/RMI2 results in the destabilization ...
In particular, the present invention provides methods for obtaining a molecule specifically binding to a target of interest, through a combinatorial mutation/selection approach with an OB-fold protein as a starting molecule. In particular, the target of interest can be of a different chemical nature...
The OB-fold is a diverse structure superfamily based on a β-barrel motif that is often supplemented with additional non-conserved secondary structures. Previous deletion mutagenesis and NMR hydrogen exchange studies of three OB-fold proteins showed that the structural stabilities of sites within the ...
The invention relates to a topical composition containing a variant of an OB-fold protein, and also to the process for preparing the same.