Tyrosine sulfation is an ubiquitous modification of proteins synthesized along the secretory pathway. It enhances protein-protein interactions and may be necessary for the bioactivity of secreted proteins and peptides. To predict tyrosine sulfation, a consensus has been proposed based on sequence compariso...
CARBOHYDRATES, LIPIDS, AND OTHER NATURAL PRODUCTS: Heparan Sulfate Oligosaccharides Require 6- O-Sulfation for Promotion of Basic Fibroblast Growth Factor ... LIPIDS, AND OTHER NATURAL PRODUCTS: Heparan Sulfate Oligosaccharides Require 6- O-Sulfation for Promotion of Basic Fibroblast Growth Factor Mitog...
Using site-directed mutagenesis, we have examined the role of residues neighboring the sulfation site of an intact protein, human progastrin,in vivo. The results show that the charge of the residue in the amino-terminal position (−1) of the tyrosine is critical and can be neutral or ...
require 6-O-sulfate to inhibit L- and P-selectin function, and in this study we observed that 6-O-sulfate is required for its interaction with CXCL12. We conclude that heparin-induced leukocytosis requires glucosamine 6-O-sulfation and is caused by blockade of L-selectin-, P-selectin-, and...
Changes in preferable conformation of the C(4)-C(5) bond caused by per-O-sulfation propyl furanosides. All the mentioned changes certainly affect the values of the 1H-1H coupling constants. To study this influence in detail, DFT/B3LYP/pcJ-1 calculation of the constants for low-energy confor...
O-glycans were released from CF and ND mucins and derivatized with 2-aminobenzamide (2-AB), separated by ion exchange chromatography, and quantified by fluorescence. There was nearly a two-fold increase in sulfation and sialylation in CF compared with ND mucin. High performance liquid ...
(15.63 ± 1.55% vs. 11.68 ± 0.56%,P < 0.01 by unpairedttest, Fig.6ainset) while no statistically different changes were observed in N- and 2-O-sulfation frequency. Sdc2 D1 domain also appeared sufficient to promote the increase in 6-O sulfation as HS chains isolated ...
and SO<SUB>2</SUB> + O<SUB>2</SUB> + H<SUB>2</SUB>O in the temperature range 300-673 K at the partial pressures of SO<SUB>2</SUB>, O<SUB>2</SUB>, and H<SUB>2</SUB>O equal to 1.5 × 10<SUP>2</SUP>, 1.5 × 10<SUP>2</SUP>, and 3 × 10<SUP>2</SUP> Pa...
Tyrosine sulfation is an ubiquitous modification of proteins synthesized along the secretory pathway, It enhances protein-protein interactions and may be necessary for the bioactivity of secreted proteins and peptides. To predict tyrosine sulfation, a consensus has been proposed based on sequence compariso...
Mutations in multiple genes required for proper O-mannosylation of α-dystroglycan are causal for congenital/limb-girdle muscular dystrophies and abnormal brain development in mammals. Previously, we and others further elucidated the functional O-mannose glycan structure that is terminated by matriglycan...