N -Acetylglucosamine-6- O -sulfotransferase (GlcNAc6ST) was cloned in the mouse and humans ( Uchimura et al. 1998b , c ). The expressed enzyme transferred sulfate to nonreducing GlcNAc but not to internally located GlcNAc. This specificity is consistent with that of the enzyme previously ...
N-Acetylglucosamine 6-O-sulfotransferase-2 (GlcNAc6ST-2) catalyzes the sulfation of mucin-like glycoproteins, which function as ligands for a lymphocyte ho... Y Tobisawa,Y Imai,M Fukuda,... - 《Journal of Biological Chemistry》 被引量: 97发表: 2011年 Characterization of an N-acetylglucosamin...
The sulfation of these ligands depends on the action of two HEV-expressed N-acetylglucosamine 6-O-sulfotransferases: GlcNAc6ST-2 and to a lesser degree GlcNAc6ST-1. Induction of PNAd has also been shown to occur in a number of human inflammatory diseases including rheumatoid arthritis (RA)....
Sulfation of this epitope on the N-acetylglucosamine (GICNAc) residue confers high-avidity L-selectin binding, and is thought to be restricted in the vasculature to sites of sustained lymphocyte recruitment. The GIcNAc-6-Osulfotransferase that installs the sulfate ester may be a key modulator of...
Sialyl 6-sulfo Lewis X is a major capping group of L-selectin ligands. N-Acetylglucosamine (GlcNAc) 6-sulfation is essential for the ligand activity, and is catalyzed by GlcNAc 6-O-sulfotransferases (GlcNAc6STs) of which GlcNAc6ST-1 and GlcNAc6ST-2 are expressed in HEV. Here, we ...
Major sulfated N-glycans were identified in both porcine and mouse PNS myelin, demonstrating that the 6-O-sulfation of N-acetylglucosamine (GlcNAc-6-O-sulfation) is highly conserved in PNS myelin between these species. P0 protein, the most abundant glycoprotein in PNS myelin and mutations in ...
N-Acetylglucosamine-6-sulfotransferase-1 (GlcNAc6ST-1) is a Golgi-resident glycoprotein that is responsible for sulfation of the l-selectin ligand on endothelial cells. Here, we report the sites at which GlcNAc6ST-1 is modified with N-linked glycans and the effects that each glycan has on ...
Sulfation of N-acetylglucosamine residues occurs by the action of N-acetylglucosaminyl-6-sulfotransferase (GlcNAc6ST), and sulfation of galactose residues is performed by keratan sulfate galactose-6-sulfotransferase (KS Gal6ST). The former enzyme transfers sulfate only to terminal N-acetylglucosamine ...
are serine-linked linear polymers elongated as binary alternation of glucuronic acid and N-acetylglucosamine2. Regions of a growing GAG chain undergo enzymatic-mediated modifications (i.e., epimerization, N-deacetylation/sulfation, and O-sulfation) that ultimately give rise to distinct sulfation patterns...
8. A “donor substrate” is a molecule recognized by, e.g., a Core-β1,6-N-acetylglucosaminyltransferase and that contributes an N-acetylglucosaminyl moiety for the transferase reaction. For C2GnT3, a donor substrate is UDP-N-acetylglucosamine. An “acceptor substrate” is a molecule, pre...