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For Pacific Biosciences sequencing, an aliquot of 15 μg DNA was spun in a Covaris g-TUBE (Covaris, Woburn, USA) at 4355 × g for 60 s using an Eppendorf 5424 centrifuge (Eppendorf, NY, USA). DNA fragments were then purified, end-repaired, and ligated with SMRTbell sequencing...
At the end of the insulin clamps, the in vivo insulin-stimulated glucose utilization index of individual tissues was determined using 2-deoxy-d-[1-3H] glucose (30 µCi/rat; Amersham Biosciences UK Ltd, Buckinghamshire, UK). A bolus of 2-deoxy-glucose was injected through the jugular ve...
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Martek Biosciences Corporation Annual Report. 2007. Available online: http://library.corporate-ir.net/library/11/116/116214/items/281192/MATK_2007_Annual.pdf (accessed on 25 February 2018). Fujitani, N.; Sakaki, S.; Yamaguchi, Y.; Takenaka, H. Inhibitory effects of microalgae on the activat...
Each 10 μL reaction consisted of 2 ng DNA, 5 μL innuMIX qPCR DSGreen Standard (Analytik Jena GmbH), 400 nM each of forward and reverse primers, and DEPC-treated water (G-Biosciences, St. Louis, MO, USA) in a 96-well plate in duplicate. The amplification comprised an initial ...