Before analysis, each chromatogram was trimmed to remove low-quality sequence. Data and statistical analysis Analysis of the resequencing of 16 pooled DNAs for each gene was performed using the Pool2 Package (Hoh et al. 2003). Based on the estimated frequencies, the Pool2 statistically ...
Structural rearrangements of the genome resulting in genic imbalance due to copy number change are often deleterious at the organismal level, but are common in immortalized cell lines and tumors, where they may be an advantage to cells. In order to explo
We have identified a novel peroxisomal targeting sequence (PTS) at the extreme COOH terminus of human catalase. The last four amino acids of this protein (... PE Purdue,PB Lazarow - 《Journal of Cell Biology》 被引量: 386发表: 1996年 Motif Refinement of the Peroxisomal Targeting Signal 1 ...
To further reveal the origin and evolution of the Rf4 locus, we re-analyzed the genome sequences of the Rf4 locus using publicly available rice genome information for ZS97B (Zhenshan97B, a maintainer line with rf4i) (http://rice.hzau.edu.cn/rice_rs3/)23, Nip (Nipponbare, a ...
When studying the population dynamics of csd alleles in honey bees, one is faced with the history of enormous an- thropogenic influence (bee management) in many parts of the world. As detailed herein, we circumvented this problem by sampling csd sequences from localiti...
Effect of location of A UUUA sequences on stability of RβG RNA. The AU-rich sequences are located in 3'UTR of transiently expressed RNAs coding for many cytokines and oncogenes.7 We placed either the AT62 or GC62 motif in the second exon (EX2.AT62; EX2.GC62) of RβG expression...
Transcription factor proteins bind specific DNA sequences to control the expression of genes. They contain DNA binding domains which belong to several super-families, each with a specific mechanism of DNA binding. The total number of transcription factor
In humans, the initiation of meiotic double-stranded breaks (DSBs) is thought to begin with the binding of the protein PRDM9 to a degenerate 13-bp sequence motif [15–17]. This motif is also enriched in CNV breakpoints [2], including several involved in disease [18], which implicates ...
transfer DNA (T-DNA) and inserted into target cells with help from virulence (vir) genes contained within the Ti plasmid, enabling tractable engineering by cloning different sequences into the T-DNA region1. The simplicity of changing the transgene target by customizing the sequence between the ...
Target fragments produced by the A genome-specific primers (TaMAF/TaMAR) were detected only in accessions with chromosome 7A (CS, T. urartu, T. dicoc- coides, NT7D7A and NT7D7B). Likewise, target fragments produced by the D genome-specific primers (TaMDF/TaMDR) were detected only in...