We present a method for estimating and providing a confidence interval for the number of DNA replication origins in the genome of the yeast Kluyveromyces lactis. The method requires an initial set of verified sites from which a position specific frequency matrix (PSFM) can be constructed. We fur...
Genome-wide estimation of firing efficiencies of origins of DNA replication from time-course copy number variation data p pBackground/p pDNA replication is a fundamental biological process during S phase of cell division. It is initiated from several hundreds of origins alon... H Luo,J Li,...
Origins of Replication Determine Plasmid Copy Number.Todd, T. EckdahlA., Malcolm CampbellLaurie, J. HeyerJeffrey, L. PoetDavid N., BlauchNicole L., SnyderDustin, T. AtchleyErich, J. BakerMicah, BrownElizabeth, C. Brunner
The copy number of a plasmid is linked to its functionality, yet there have been few attempts to optimize higher-copy-number mutants for use across diverse origins of replication in different hosts. We use a high-throughput growth-coupled selection assay and a directed evolution approach to rapid...
DNA replication is largely regulated at the level of initiation when the origin recognition complex (ORC) binds to cis-acting origins of replication and together with Cdc6 and Cdt1/Dup loads the replicative helicase (Bell and Kaguni, 2013). Subsequent activation of the helicase results in the ...
Here, we show that the number of MCMs loaded at origins regulates replication timing. We show for the first time in vivo that multiple MCMs are loaded at origins. Because early origins have more MCMs loaded, they are, on average, more likely to fire early in S phase. Our results ...
Replication timing is regulated by the number of MCMs loaded at origins Replication timing is a crucial aspect of genome regulation that is strongly correlated with chromatin structure, gene expression, DNA repair, and genome e... SP Das,T Borrman,VWT Liu,... - 《Genome Research》 被引量:...
origins and the functional significance of CNVs and review non-NGS CNV detection technologies. Considerations of CNV screening across various clinical NGS contexts are discussed, including candidate gene,exome, whole genome, and cell-freeDNA sequencingapplications. The main conceptual approaches to CNV ...
Copy number variation (CNV) can drive rapid evolution in changing environments. In microbial pathogens, such adaptation is a key factor underpinning epidemics and colonization of new niches. However, the genomic determinants of such adaptation remain poo
Variations in DNA copy number carry information on the modalities of genome evolution and mis-regulation of DNA replication in cancer cells. Their study can help localize tumor suppressor genes, distinguish different populations of cancerous cells, and identify genomic variations responsible for disease ...