At the beginning of replication, DNA is unzipped by helicase (yellow). Then primase (red) creates the first few base pairs that DNA polymerase (blue) will recognize, before it begins replication.DNA replicationScitable
CHO cells were pulse labeled with 3 H-thymidine after synchronization and blockage at the beginning of S phase for various intervals. The distribution of initiation sites for DNA replication and rates of chain growth were measured in autoradiographs prepared from these cells. Origins used for replic...
The ability of cells to respond to DNA damage and to stalled DNA replication forks is essential for preventing genome instability, cancer, and cell death. DNA-repair pathways recognize and repair specific subsets of DNA lesions. They use defined damage-recognition proteins to sense specific lesions...
The initiation of DNA replication in eukaryotic cells at the onset of S phase requires the origin recognition complex (ORC) [1]. This six-subunit complex, ... AK Bielinsky,H Blitzblau,EL Beall,... - 《Current Biology》 被引量: 145发表: 2001年 加载更多研究点推荐 Eukaryotes ...
In mice, the first round of DNA replication occurs in fertilized eggs (1-cell embryos), while the onset of zygotic gene transcription begins 20 hours after fertilization, a time that normally coincides with formation of a 2-cell embryo. One approach to investigating the mechanisms that control ...
aIn order to initiate the process of replication of the DNA, two informing sequence codes denominated primers are required which promote the beginning and reversion of the reaction of the polymerase (RNA-pol) at particular locations of the genome. 为了创始脱氧核糖核酸的复制的过程,要求二通知的序列...
aMay not be the first phase of a people from the white May not be the first phase of a people from the white[translate] a三座教学楼,一座实验楼,一座办公室 Three classroom buildings, an experimental building, an office[translate] aone enriched in DNA replication and the other in[translate]...
Thus, although the ensuing steps of reverse transcription are common to these elements, several variations in which the replication primer is used have been exploited. In addition, the PBS of the viral RNA genome can vary in size from an 11 nt sequence, through a bipartite cis-acting element...
Telomeres are the protein-DNA structures at the ends of eukaryotic chromosomes. In yeast, and probably most other eukaryotes, telomeres are essential. They allow the cell to distinguish intact from broken chromosomes, protect chromosomes from degradation, and are substrates for novel replication mechanis...
aDNA sequencing mimics the basic process used to copy DNA in a cell during chromosomal replication, except that the procedure is done in a tube or microtiter plate using a minimal set of components. Most DNA sequencing techniques require that there be a "template", (i.e., a biological samp...