NUCLEAR RUN-ON TRANSCRIPTION Reagents:1.50X Denhardt’s solution: 1% Ficoll, 1% polyvinylpyrrolidone, and 1% bovine serum albumin (BSA). Filter sterilize.2.Dnase I 3.Hybridization buffer: 50% formamide, 6X SSC, 10X Denhardt’s solution, 0.2% SDS 4.Lysis buffer: 10mM NaCl, 3mM MgCl2...
网络转录分析 网络释义 1. 转录分析 又如进行中的转录分析(nuclear run on transcription assay)时,在体外将细胞核分离出来,然后在α -32P-ATP 的存 在下进行转 … www.docin.com|基于2个网页
Nuclear “run-on” (or “run-off”) transcription assays have been used to obtain quantitative information about the relative rates of transcription of different genes in nuclei isolated from a particular tissue or organ. This information can then be used in analyzing the factors that control the...
The nuclear run-on and other experiments suggest that RNA polymerase II-dependent transcription is inhibited before RNA polymerase I-dependent transcription. This conclusion is also supported by Northern analyses of selected mRNAs in nonsynchronized and synchronized cells after a shift to the non...
NuclearRun-OnAssay StephenT.Smale INTRODUCTION Thenuclearrun-onassaywasdevelopedasamethodforestablishingthatthetranscriptioninitiation ratecontributestotheregulatedexpressionofmammaliangenes.Thedifferencebetweenmonitor- inggeneexpressionbythenuclearrun-onassayversusmostotherassaysisthatthenuclearrun-on assayprovidesameasur...
"nuclear run-on assay(=run-ontranscription assay)" Chinese translation ("nuclear run-on assay(=run-ontranscription assay)" in Chinese, "nuclear run-on assay(=run-ontranscription assay)" 中文翻译, "nuclear run-on assay(=run-ontranscription assay)"发音): ...
To the nuclei add100 ul of 2 Xtranscription buffer (20 mM Tris-HCl, pH7.4, 200 mM KCl, 5 mM MgCl2, 4 mM each ofATP, GTP and CTP, 200 mM sucrose, 4 mM dithiothreitol and 20% glycerol) and 8 ul of10 mM biotin-16-UTP (Roche), gently mix and incubatefor30 min at 29°C. To...
The nuclear run-on and other experiments suggest that RNA polymerase II-dependent transcription is inhibited before RNA polymerase I-dependent transcription. This conclusion is also supported by Northern analyses of selected mRNAs in nonsynchronized and synchronized cells after a shift to the non...
1989 . Neuropeptide gene transcription in central and peripheral nervous system tissue by nuclear run-on assay . Methods Enzymol 168 : 681 – 690 .White JD, LaGamma EF. 1989. Neuropeptide gene transcription in central and peripheral nervous system tissue by nuclear run-on assay. Methods Enzymol...
Trumbore MW,Berger SL.Prothymosin alpha is a nonspecific facilitator of nuclear processes:studies of run-on transcription.Protein Expression and Purification. 2000Trumbore MW,Berger SL.Prothymosin alpha is a nonspecific facilitator of nuclear processes: studies of run-on transcription. Protein Expression...