Nuclear run on transcription assay NUCLEAR RUN-ON TRANSCRIPTION Reagents:1.50X Denhardt’s solution: 1% Ficoll, 1% polyvinylpyrrolidone, and 1% bovine serum albumin (BSA). Filter sterilize.2.Dnase I 3.Hybridization buffer: 50% formamide, 6X SSC, 10X Denhardt’s solution, 0.2% SDS 4....
网络转录分析 网络释义 1. 转录分析 又如进行中的转录分析(nuclear run on transcription assay)时,在体外将细胞核分离出来,然后在α -32P-ATP 的存 在下进行转 … www.docin.com|基于2个网页
Mini-scale method for nuclear run-on transcription assay in - Kanazawa, O’Dell, et al. - 2000 () Citation Context ... “m”: AclI (AACmGTT), BsaBI (GAmTNNNNAmTC), BsiEI (CmGRYCmG), ClaI (ATCmGAT), and HpaII (CCmGG). Run-on assays. Nuclei from cold-treated sporangia were ...
FIGURE 1. Nuclear run-on transcription assay overview. The nuclear run-on technique (Derman et al. 1981; Greenberg and Ziff 1984; Powell et al. 1984; Ucker and Yamamoto 1984) is typically performed with cell populations that express
White, J.D. and LaGamma, E.F., Neuropeptide gene transcription in central and peripheral nervous system tissues by nuclear run-on assay, Methods Enzymol., 168 (1989) 681-690.White JD , LaGamma EF . 1989 . Neuropeptide gene transcription in central and peripheral nervous system tissue by ...
RNA integrity and quantity were measured on 2100 Bioanalyzer (Agilent Technologies, USA). After sample QC, library construction was performed using NEBNext Ultra II RNA Library Prep Kit (Illumina, USA) according to manufacturer’s protocols. For the first batch, the libraries were run with BGI ...
(1998) Measurements of rate of transcription in isolated nuclei by nuclear `run-off' assay. Methods Mol. Biol., 86, 201-207.Srivastava RAKS G.: Measurements of rate of transcription in isolated nuclei by nuclear "run-off" assay. The Nucleic Acid Protocol Handbook (Edited by: Rapley R)....
The primary mechanism of cytochrome P450 induction is via increased gene transcription which typically occurs through nuclear receptor activation. The most common nuclear receptors involved in the induction of drug metabolizing enzymes include the pregnane X receptor (PXR), the aryl hydrocarbon receptor (...
YAP-MAMLD1 concentrates transcription factors and co-activators, including BRD4, MED1 and TEAD, in condensates while excluding transcriptional repressive PRC2, and induces long-range enhancer–promoter interactions that promote transcription and oncogenic programmes. Blocking condensate-mediated transcriptional...
2C). To determine if the HAS plays a role in ErbB2-regulated DEPTOR transcription, we first constructed a luciferase reporter under the control of DEPTOR promoter containing HAS (DEPTOR-Luc). We then performed luciferase reporter assay and found that ErbB2 silencing relieved the inhibition of ...