Nuclear run on transcription assay NUCLEAR RUN-ON TRANSCRIPTION Reagents:1.50X Denhardt’s solution: 1% Ficoll, 1% polyvinylpyrrolidone, and 1% bovine serum albumin (BSA). Filter sterilize.2.Dnase I 3.Hybridization buffer: 50% formamide, 6X SSC, 10X Denhardt’s solution, 0.2% SDS 4....
网络转录分析 网络释义 1. 转录分析 又如进行中的转录分析(nuclear run on transcription assay)时,在体外将细胞核分离出来,然后在α -32P-ATP 的存 在下进行转 … www.docin.com|基于2个网页
Nuclear run-on assays: assessing transcription by measuring density of engaged RNA polymerases. Methods Enzymol - Hirayoshi, Lis - 1999 () Citation Context ...ation of the indirect TR data calculated from RS and RA (see below). For a long time, the transcription run-on...
"nuclear run-on assay(=run-ontranscription assay)" Chinese translation ("nuclear run-on assay(=run-ontranscription assay)" in Chinese, "nuclear run-on assay(=run-ontranscription assay)" 中文翻译, "nuclear run-on assay(=run-ontranscription assay)"发音): ...
NuclearRun-OnAssay StephenT.Smale INTRODUCTION Thenuclearrun-onassaywasdevelopedasamethodforestablishingthatthetranscriptioninitiation ratecontributestotheregulatedexpressionofmammaliangenes.Thedifferencebetweenmonitor- inggeneexpressionbythenuclearrun-onassayversusmostotherassaysisthatthenuclearrun-on assayprovidesameasur...
White, J.D. and LaGamma, E.F., Neuropeptide gene transcription in central and peripheral nervous system tissues by nuclear run-on assay, Methods Enzymol., 168 (1989) 681-690.White JD , LaGamma EF . 1989 . Neuropeptide gene transcription in central and peripheral nervous system tissue by ...
Glucocorticosteroids increase beta 2-adrenergic receptor transcription in human lung The stability of β2R mRNA was unchanged by Dex, and a nuclear run-on assay revealed that Dex approximately doubled the transcriptional rate of the β... JC Mak,M Nishikawa,PJ Barnes - 《Am J Physiol》 被引...
Using a nuclear runoff transcription assay, we demonstrated that Ang II stimulated the transcription rate of the c-fos gene. This activation of c-fos ... AJ Naftilan,RE Pratt,CS Eldridge,... - 《Hypertension》 被引量: 693发表: 1989年 ...
Inhibition of Transcription of Herpes Simplex Virus Immediate Early Genes in Interferon-treated Human Cells Using the nuclear run-off transcription assay, we showed that IFN selectively inhibited transcription of the HSV-1 genes, with no effect on transcription ... F Oberman,A Panet - 《Journal ...
RNA integrity and quantity were measured on 2100 Bioanalyzer (Agilent Technologies, USA). After sample QC, library construction was performed using NEBNext Ultra II RNA Library Prep Kit (Illumina, USA) according to manufacturer’s protocols. For the first batch, the libraries were run with BGI ...