必应词典为您提供nuclear-run-on-assay的释义,网络释义: 核连缀分析;进行性核连缀转录分析;核转录活性测定;
Nuclei Run-on是一种用于检测细胞内正在转录的mRNA的技术。其原理是通过迅速冷冻细胞核，使正在转录的mRNA复合体处于冻结状态，随后加入ATP、GTP、CTP和32P-UTP，重新启动并终止mRNA的转录，从而标记冻结时正在转录的mRNA。提取mRNA后，通过cDNA探针和对照cDNA标记尼龙膜检测目标mRNA。细胞核的提取过程包括：...
我想问一下关于nuclear run-on assay的原理以及其与Northern blot的比较,核连缀实验, 答案 Northern印迹杂交(Northern blot).原理:将RNA固定于尼龙膜上以DNA探针识别待检mRNA这是一种将RNA从琼脂糖凝胶中转印到硝酸纤维素膜上的方法.DNA印迹技术由Southern于1975年创建,称为Southern印迹技术,RNA印迹技术...相关推荐...
The nuclear run-on assay measures the relative in situ transcription rate of specific genes in intact nuclei ( 1 , 2 ). It provides information on the synthesis of a specific gene that occurs as a function of cell state, as opposed to a change in mRNA degradation or transport from the ...
Nuclei Run-on 原理：迅速冷冻，使细胞核内正在转录的mRNA复合体处于冻结状态 加入ATP/GTP/CTP/32P-UTP，重新恢复mRNA的转录并使之结束（标记冻结时正在转录的mRNA)，提取mRNA 以cDNA探针及对照cDNA标记尼龙膜后检测目标mRNA 2、操作过程：细胞核的提取 处理细胞（培养于10厘米培养皿）吸去培养液，迅速...
NuclearRun-OnAssay StephenT.Smale INTRODUCTION Thenuclearrun-onassaywasdevelopedasamethodforestablishingthatthetranscriptioninitiation ratecontributestotheregulatedexpressionofmammaliangenes.Thedifferencebetweenmonitor- inggeneexpressionbythenuclearrun-onassayversusmostotherassaysisthatthenuclearrun-on assayprovidesameasur...
"nuclear run-on assay(=run-ontranscription assay)" Chinese translation ("nuclear run-on assay(=run-ontranscription assay)" in Chinese, "nuclear run-on assay(=run-ontranscription assay)" 中文翻译, "nuclear run-on assay(=run-ontranscription assay)"发音): ...
Nuclear run on transcription assay NUCLEAR RUN-ON TRANSCRIPTION Reagents:1.50X Denhardt’s solution: 1% Ficoll, 1% polyvinylpyrrolidone, and 1% bovine serum albumin (BSA). Filter sterilize.2.Dnase I 3.Hybridization buffer: 50% formamide, 6X SSC, 10X Denhardt’s solution, 0.2% SDS 4....
我想问一下关于nuclear run-on assay的原理以及其与Northern blot的比较,核连缀实验, 扫码下载作业帮搜索答疑一搜即得 答案解析 查看更多优质解析解答一 举报 Northern印迹杂交(Northern blot).原理:将RNA固定于尼龙膜上以DNA探针识别待检mRNA这是一种将RNA从琼脂糖凝胶中转印到硝酸纤维素膜上的方法.DNA印迹技术由...
The stability of β2R mRNA was unchanged by Dex, and a nuclear run-on assay revealed that Dex approximately doubled the transcriptional rate of the β... JC Mak,M Nishikawa,PJ Barnes - 《Am J Physiol》 被引量: 642发表: 1995年 Cardiotrophin-1 Activates a Distinct Form of Cardiac Muscle...